Strain Data Sheet

RBRC02892

Strain Information

Image
BRC No.RBRC02892
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(FucciS/G2/M)#504Bsi Tg(FucciG1)#596Bsi
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain descriptionTransgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenance#504: Carrier x Carrier (Homozygote x Homozygote); #596 [Carrier x Noncarrier]
References
Structure and expression of the duck alpha-enolase/tau-crystallin-encoding gene.
Kim R Y, Lietman T, Piatigorsky J, Wistow G J
Gene, 108, 193-200 (1991). 1889745

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A
Cell, 132, 487-498 (2008). 18267078

Preferential localization of IgG memory B cells adjacent to contracted germinal centers.
Aiba Y, Kometani K, Hamadate M, Moriyama S, Sakaue-Sawano A, Tomura M, Luche H, Fehling H J, Casellas R, Kanagawa O, Miyawaki A, Kurosaki T
Proc. Natl. Acad. Sci. USA, 107(27)12192-12197(2010). 20547847

Health Report

Examination Date / Room / Rack2025/01/20Room:3-CRack:CSentinel mouse program
2024/10/21Room:3-CRack:CSentinel mouse program
2024/07/22Room:3-CRack:CSentinel mouse program
2024/04/22Room:3-CRack:CSentinel mouse program
2024/01/22Room:3-CRack:CSentinel mouse program
2023/10/23Room:3-CRack:CSentinel mouse program
2023/07/24Room:3-CRack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
CDT1 chromatin licensing and DNA replication factor (human)UNCDT1
GMNN geminin (human)UNGMNN
mAG monomeric Azami Green (Coral)UNmAG
mKO2 monomeric Kusabira Orange (Coral)UNmKO2

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Coral mKO2 (monomeric Kusabira Orange) cDNA, human CDT1 cDNA, Coral mAG(monomeric Azami Green) cDNA, human Geminin cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 132, 487-498 (2008). Gene, 108, 193-200 (1991).Distribution of the BIOLOGICAL RESOURCE is limited to for academic researches in not-for-profit entities. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from MBL, prior to distribution.
    DepositorMEDICAL ＆ BIOLOGICAL LABORATORIES CO., LTD. (Medical ＆ Biological Laboratories Co., Ltd.)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Miyawaki Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Mar 2009

    S_G2_M-Green.dna
    G1-Red.dna

    BRC mice in Publications

    El Azhar Y, Schulthess P, van Oostrom MJ, Weterings SDC, Meijer WHM, Tsuchida-Straeten N, Thomas WM, Bauer M, Sonnen KF.
    Unravelling differential Hes1 dynamics during axis elongation of mouse embryos through single-cell tracking.
    Development 151(18) (2024) 39315665
    Takata K, Kozaki T, Lee CZW, Thion MS, Otsuka M, Lim S, Utami KH, Fidan K, Park DS, Malleret B, Chakarov S, See P, Low D, Low G, Garcia-Miralles M, Zeng R, Zhang J, Goh CC, Gul A, Hubert S, Lee B, Chen J, Low I, Shadan NB, Lum J, Wei TS, Mok E, Kawanishi S, Kitamura Y, Larbi A, Poidinger M, Renia L, Ng LG, Wolf Y, Jung S, Önder T, Newell E, Huber T, Ashihara E, Garel S, Pouladi MA, Ginhoux F.
    Induced-Pluripotent-Stem-Cell-Derived Primitive Macrophages Provide a Platform for Modeling Tissue-Resident Macrophage Differentiation and Function.
    Immunity 47(1) 183-198.e6(2017) 28723550
    Sakitani K, Hayakawa Y, Deng H, Ariyama H, Kinoshita H, Konishi M, Ono S, Suzuki N, Ihara S, Niu Z, Kim W, Tanaka T, Liu H, Chen X, Tailor Y, Fox JG, Konieczny SF, Onodera H, Sepulveda AR, Asfaha S, Hirata Y, Worthley DL, Koike K, Wang TC.
    CXCR4-expressing Mist1+ progenitors in the gastric antrum contribute to gastric cancer development.
    Oncotarget 8(67) 111012-111025(2017) 29340033
    Imayoshi I, Isomura A, Harima Y, Kawaguchi K, Kori H, Miyachi H, Fujiwara T, Ishidate F, Kageyama R.
    Oscillatory control of factors determining multipotency and fate in mouse neural progenitors.
    Science 342(6163) 1203-8(2013) 24179156