Strain Data Sheet

RBRC02945

Strain Information

Image
BRC No.RBRC02945
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(Thy1-ECFP,-EYFP)1Hsak
Former Common nameThy1-stop-syn::ECFP-ires-gEYFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ko Kobayakawa, Reiko Hayashi and Hitoshi Sakano, School of Science The University of Tokyo in 2002. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs.
Strain descriptionThy1-stop-syn::CFP-ires-gapEYFP mice having a transgene of a Thy1 promoter, loxP-STOP loxP cassette, synaptotagmin-CFP and ires-gapEYFP. Tissue-specific expression of Cre recombinase has not been examined yet.
Colony maintenanceCarrier x Noncarrier [or Crossing to Crlj:CD1(ICR)]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ECFP Enhanced Cyan Fluorescent Protein (Aequorea victoria)UNECFP mouse Thy1 promoter
EYFP Enhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP
Gap43 mouse gap43 palmitoylation signalUNGap43
His3 His3 (S. cerevisiae)UNHis3
IRES internal ribosomal entry site (EMCV)UN
loxP phage P1 loxPUNloxP
mouse synaptotagmin I (Syt1)UN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Thy1 promoter, P1 phage loxP site, S. cerevisiae His3, Jellyfish ECFP, EYFP, encephalomyocarditis virus ires (internal ribosome entry site, Mouse gap43 palmitoylation signal, Mouse synaptotagmin I (Syt1)
    Research applicationCell Biology Research
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER (Ko KOBAYAKAWA : skobayak@mail.ecc.u-tokyo.ac.jp). The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in first publication of the results obtained from the use of the BIOLOGICAL RESOURCE.
    DepositorHitoshi Sakano (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data