Strain Data Sheet

RBRC02958

Strain Information

Image
BRC No.RBRC02958
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(tetO-EGFP/TeNT)1Nak
Former Common nameTeNT
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Mutsuya Yamamoto and Shigetada Nakanishi in 2003.
Strain descriptionThis transgenic mice express the EGFP and tetanus neurotoxin light chain gene under the control of the tetracycline operator sequence.
Colony maintenanceHemizygote x C57BL/6 [C57BL/6JJmsSlc]
References
Reversible suppression of glutamatergic neurotransmission of cerebellar granule cells in vivo by genetically manipulated expression of tetanus neurotoxin light chain.
Yamamoto M, Wada N, Kitabatake Y, Watanabe D, Anzai M, Yokoyama M, Teranishi Y, Nakanishi S
J. Neurosci., 23, 6759-6767 (2003). 12890769

Conditioned eyeblink learning is formed and stored without cerebellar granule cell transmission.
Wada N, Kishimoto Y, Watanabe D, Kano M, Hirano T, Funabiki K, Nakanishi S
Proc. Natl. Acad. Sci. USA, 104, 16690-16695 (2007). 17923666

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)UN E. coli bidirectional TRE promoter (tetracycline responsive promoter)
Odc1 mouse ornithine decarboxylase (PEST sequence) cDNAUNOdc1
TeNT tetanus neurotoxin light chain (Clostridium tetani)UNTeNT

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNATetracycline response element-CMV promoter, Aequorea victoria EGFP, Clostridium tetani TeNT light chain, mouse PEST sequence (proline, glutamic acid, serine, and threonine) of ornithine decarboxylase (residues 1402-1524), rabbit beta-globin poly A signal
    Research applicationFluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 23, 6759-6767 (2003). Proc. Natl. Acad. Sci. USA, 104, 16690-16695 (2007). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShigetada Nakanishi (Osaka Bioscience Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Niwa Y, Kanda GN, Yamada RG, Shi S, Sunagawa GA, Ukai-Tadenuma M, Fujishima H, Matsumoto N, Masumoto KH, Nagano M, Kasukawa T, Galloway J, Perrin D, Shigeyoshi Y, Ukai H, Kiyonari H, Sumiyama K, Ueda HR.
    Muscarinic Acetylcholine Receptors Chrm1 and Chrm3 Are Essential for REM Sleep.
    Cell Rep 24(9) 2231-2247.e7(2018) 30157420