Strain Data Sheet

RBRC03072

Strain Information

Image
BRC No.RBRC03072
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Sohlh2<tm1Miya>
Former Common nameSohlh2 knockout mouse
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Sohlh2 affects differentiation of KIT positive oocytes and spermatogonia.
Toyoda S, Miyazaki T, Miyazaki S, Yoshimura T, Yamamoto M, Tashiro F, Yamato E, Miyazaki J
Dev. Biol., 325, 238-248 (2009). 19014927

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRES internal ribosomal entry site (EMCV)3
Sohlh2
MGI:1921684		spermatogenesis and oogenesis specific basic helix-loop-helix 23Sohlh2<tm1Miya>
MGI:3829597		targeted mutation 1, Jun-ichi Miyazaki
lacZ beta-galactosidase (E. coli)3
neo neomycin resistance gene (E. coli)3
puro puromycin acyltransferase (Streptomyces alboniger)3puro mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal oogenesis(MP:0001931)

  • abnormal spermatocyte morphology(MP:0006379)

  • abnormal spermatogonia morphology(MP:0006378)

  • absent ovarian follicles(MP:0002777)

  • female infertility(MP:0001926)
    • more 4 phenotypes
  • impaired ovarian folliculogenesis(MP:0001129)

  • male infertility(MP:0001925)

  • ovary atrophy(MP:0004833)

  • testicular atrophy(MP:0003205)
    • Detailed phenotype data

    Ordering Information

    Donor DNAIRES-beta geo-Pgk-puro
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 325, 238-248 (2009).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorJun-ichi Miyazaki (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data