Strain Data Sheet

RBRC03099

Strain Information

Image
BRC No.RBRC03099
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-EGFP/MIR145/MIR143)B
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takashi Iwamoto, Department of Biomedical Sciences, Chubu Universiy in 2006.
Strain descriptionThis strain overexpresses miR-143 and miR-145 in many tissues. Thier expression can be monitored by GFP. miR-143 and miR-145 are possible tumor suppressor genes and miR-143 has been reported to be upreglated during adipocyte differentiation.
Colony maintenance
ReferencesJ. Blol. Chem., 279, 52361-52365 (2004). 15504739
Biochem. Biophys. Res. Commun., 372, 856-861 (2008). 18533106
Mol. Cancer. Res., 1, 882-891 (2003). 14573789

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
MIR143miR-143UNMIR143

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
MIR145miR-145UNMIR145

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Homo sapiens pri-miR-145, Homo sapiens pri-miR-143, Aequrea Victoria EGFP
Research application
Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. All data got with the BIOLOGICAL RESOURCE must be published as a joint result with the DEPOSITER.
DepositorTakashi Iwamoto (Chubu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data