Strain Data Sheet

RBRC03099

Strain Information

Image
BRC No.RBRC03099
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-EGFP/MIR145/MIR143)B
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takashi Iwamoto, Department of Biomedical Sciences, Chubu Universiy in 2006.
Strain descriptionThis strain overexpresses miR-143 and miR-145 in many tissues. Thier expression can be monitored by GFP. miR-143 and miR-145 are possible tumor suppressor genes and miR-143 has been reported to be upreglated during adipocyte differentiation.
Colony maintenance
References
Reduced accumulation of specific microRNAs in colorectal neoplasia.
Michael M Z, O' Connor S M, van Holst Pellekaan N G, Young G P, James R J
Mol. Cancer. Res., 1, 882-891 (2003). 14573789

MicroRNA-143 regulates adipocyte differentiation.
Esau C, Kang X, Peralta E, Hanson E, Marcusson E G, Ravichandran L V, Sun Y, Koo S, Perera R J, Jain R, Dean N M, Freier S M, Bennett C F, Lollo B, Griffey R
J. Blol. Chem., 279, 52361-52365 (2004). 15504739

Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Tsutsui M, Hasegawa H, Adachi K, Miyata M, Huang P, Ishiguro N, Hamaguchi M, Iwamoto T
Biochem. Biophys. Res. Commun., 372, 856-861 (2008). 18533106

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)UN CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
MIR143 miR-143UNMIR143
MIR145 miR-145UNMIR145

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal intestine morphology(MP:0000477)

  • decreased tumor growth/size(MP:0003447)

  • increased colon adenoma incidence(MP:0002044)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Homo sapiens pri-miR-145, Homo sapiens pri-miR-143, Aequrea Victoria EGFP
    Research application
    Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. All data got with the BIOLOGICAL RESOURCE must be published as a joint result with the DEPOSITER.
    DepositorTakashi Iwamoto (Chubu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data