Strain Data Sheet

RBRC03116

Strain Information

Image
BRC No.RBRC03116
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-SCAT3)43Miur
Former Common nameCAG-SCAT3
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yoshifumi Yamagochi and Masayuki Miura, Graduate School of Pharmaceutical Sciences in 2009.
Strain description
Colony maintenance
References
Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects.
Takemoto K, Nagai T, Miyawaki A, Miura M
J. Cell. Biol., 160(2), 235-243 (2003). 12527749

Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure.
Yamaguchi Y, Shinotsuka N, Nonomura K, Takemoto K, Kuida K, Yosida H, Miura M
J. Cell Biol., 195(6), 1047-1060 (2011). 22162136

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
CFP Cyan Fluorescent Protein (Aequorea victoria)UNCFP
SCAT3 sensor for activated caspases based on FRETUNSCAT3 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
Venus Yellow Fluorescent Protein (Aequorea victoria)UNVenus

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal neural tube closure(MP:0003720)
    • Detailed phenotype data

    Ordering Information

    Donor DNASCAT3 (Jellyfish ECFP cDNA, caspase-3 cleavage site, Jellyfish Venus cDNA), chicken HS4 insulator, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Biol., 195(6), 1047-1060 (2011). The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorMasayuki Miura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data