Strain Data Sheet

RBRC03162

Strain Information

Image
BRC No.RBRC03162
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Th-EGFP)6-7Koba
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.
Strain descriptionThis transgenic strain expresses GFP under the control of rat tyrosine hydroxylase gene promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development.
Matsushita N, Okada H, Yasoshima Y, Takahashi K, Kiuchi K, Kobayashi K
J. Neurochem., 82, 295-304 (2002). 12124430

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)UN rat tyrosine hydroxylase (TH) promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNArat tyrosine hydroxylase (TH) promoter, rabbit beta-globin intron, jellyfish green fluorescent protein (GFP) cDNA, rabbit beta-globin poly A signal, SV40 early gene poly A signal
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 82, 295-304 (2002).
    DepositorKazuto Kobayashi (Fukushima Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data