Strain Data Sheet

RBRC03163

Strain Information

Image
BRC No.RBRC03163
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6J.129P2-Hmox1<tm1Mym>
Former Common nameHO-1 floxed mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Masayuki Yamamoto, Center for Tsukuba Advanced Research Alliance, Tsukuba University in 2001. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 (C57BL/6JJcl and C57BL/6JJmsSlc).
Strain descriptionHmox1 gene floxed mice. Exon 3 to 5 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
References
Hepatocyte-specific deletion of heme oxygenase-1 disrupts redox homeostasis in basal and oxidative environments.
Mamiya T, Katsuoka F, Hirayama A, Nakajima O, Kobayashi A, Maher J M, Matsui H, Hyodo I, Yamamoto M, Hosoya T
Tohoku J. Exp. Med., 216, 331-339 (2008). 19060448

Health Report

Examination Date / Room / Rack2025/01/20Room:3-CRack:CSentinel mouse program
2024/10/21Room:3-CRack:CSentinel mouse program
2024/07/22Room:3-CRack:CSentinel mouse program
2024/04/22Room:3-CRack:CSentinel mouse program
2024/01/22Room:3-CRack:CSentinel mouse program
2023/10/23Room:3-CRack:CSentinel mouse program
2023/07/24Room:3-CRack:CSentinel mouse program
2023/04/24Room:3-CRack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
DsRed2 Red Fluorescent Protein (Discosoma sp.)8DsRed2
Hmox1
MGI:96163		heme oxygenase 18Hmox1<tm1Mym> targeted mutation 1, Masayuki Yamamoto
  • COPD, severe early onset(MedGEN)

  • heme oxygenase 1 deficiency(MedGEN)
    • loxP phage P1 loxP8loxP
    loxP phage P1 loxP8loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage loxP sites, chimeric intron and splicing acceptor (from pRL-null), Discosoma sp. DsRed2 cDNA, SV40 polyA signal, mouse Heme oxygenase-1 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Tohoku J. Exp. Med., 216, 331-339 (2008). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for TWO years after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    DepositorMasayuki Yamamoto (Tohoku University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: TBUSAMTA Nonprofit
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Kaiser S, Fritsch A, Jakob L, Schallner N.
    Severity of Repetitive Mild Traumatic Brain Injury Depends on Microglial Heme Oxygenase-1 and Carbon Monoxide.
    Eur J Neurosci 61(2) e16666(2025) 39844588
    Henrich L, Kiessling I, Steimer M, Frase S, Kaiser S, Schallner N.
    Circadian dependency of microglial heme oxygenase-1 expression and inflammation determine neuronal injury in hemorrhagic stroke.
    J Inflamm (Lond) 20(1) 43(2023) 38104143
    Di Pietro C, Öz HH, Zhang PX, Cheng EC, Martis V, Bonfield TL, Kelley TJ, Jubin R, Abuchowski A, Krause DS, Egan ME, Murray TS, Bruscia EM.
    Recruitment of monocytes primed to express heme oxygenase-1 ameliorates pathological lung inflammation in cystic fibrosis.
    Exp Mol Med 54(5) 639-652(2022) 35581352
    Alves de Souza RW, Gallo D, Lee GR, Katsuyama E, Schaufler A, Weber J, Csizmadia E, Tsokos GC, Koch LG, Britton SL, Wisløff U, Brum PC, Otterbein LE.
    Skeletal muscle heme oxygenase-1 activity regulates aerobic capacity.
    Cell Rep 35(3) 109018(2021) 33882313
    Fernández-Mendívil C, Luengo E, Trigo-Alonso P, García-Magro N, Negredo P, López MG.
    Protective role of microglial HO-1 blockade in aging: Implication of iron metabolism.
    Redox Biol 38 101789(2021) 33212416
    Schallner N, Pandit R, LeBlanc R 3rd, Thomas AJ, Ogilvy CS, Zuckerbraun BS, Gallo D, Otterbein LE, Hanafy KA.
    Microglia regulate blood clearance in subarachnoid hemorrhage by heme oxygenase-1.
    J Clin Invest 125(7) 2609-25(2015) 26011640
    Parada E, Buendia I, Navarro E, Avendaño C, Egea J, López MG.
    Microglial HO-1 induction by curcumin provides antioxidant, antineuroinflammatory, and glioprotective effects.
    Mol Nutr Food Res 59(9) 1690-700(2015) 26047311
    Parada E, Egea J, Buendia I, Negredo P, Cunha AC, Cardoso S, Soares MP, López MG.
    The microglial α7-acetylcholine nicotinic receptor is a key element in promoting neuroprotection by inducing heme oxygenase-1 via nuclear factor erythroid-2-related factor 2.
    Antioxid Redox Signal 19(11) 1135-48(2013) 23311871