Strain Information | |
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Image | |
BRC No. | RBRC03327 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Ttll1<tm1Seto>/SetoRbrc |
Former Common name | TL1 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Ikegami and Mitsutoshi Setou, Mitsubishi Kagaku Institute of Life Sciences. 129 derived ES cells were used. The mice were crossed to C57BL/6J. |
Strain description | Ttll1 (TL1) gene knockout mice. Exons 2, 3 and a part of exon 4 were replaced with neo cassette. Initiation codon is in exon 4. null mutant. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Proc. Natl. Acad. Sci. USA, 107, 10490-10495 (2010). 20498047 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)15neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV PolyA signal, mouse TL1 genomic DNA |
Research application | |
Specific Term and Conditions | The BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization. The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation. The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution. |
Depositor | Mitsutoshi Setou (Mitsubishi Kasei Institute of Life Sciences) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Wu HY, Rong Y, Bansal PK, Wei P, Guo H, Morgan JI. TTLL1 and TTLL4 polyglutamylases are required for the neurodegenerative phenotypes in pcd mice. PLoS Genet 18(4) e1010144(2022) 35404950 |