Strain Data Sheet

RBRC03327

Strain Information

Image
BRC No.RBRC03327
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Ttll1<tm1Seto>/SetoRbrc
Former Common nameTL1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ikegami and Mitsutoshi Setou, Mitsubishi Kagaku Institute of Life Sciences. 129 derived ES cells were used. The mice were crossed to C57BL/6J.
Strain descriptionTtll1 (TL1) gene knockout mice. Exons 2, 3 and a part of exon 4 were replaced with neo cassette. Initiation codon is in exon 4. null mutant.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Tubulin polyglutamylation is essential for airway ciliary function through the regulation of beating asymmetry.
Ikegami K, Sato S, Nakamura K, Ostrowski L E, Setou M
Proc. Natl. Acad. Sci. USA, 107, 10490-10495 (2010). 20498047

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ttll1
MGI:2443047		tubulin tyrosine ligase-like 115Ttll1 <tm1Seto>
MGI:4461777		targeted mutation 1, Mitsutoshi Setou
neo neomycin resistance gene (E. coli)15 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal airway resistance(MP:0002328)

  • abnormal mucociliary clearance(MP:0001947)

  • abnormal respiratory motile cilium morphology(MP:0011050)

  • abnormal respiratory motile cilium physiology(MP:0011055)

  • abnormal respiratory sounds(MP:0001950)
    • more 7 phenotypes
  • abnormal tracheal ciliated epithelium morphology(MP:0002285)

  • coughing(MP:0031088)

  • immotile respiratory cilia(MP:0011649)

  • rhinosinusitis(MP:0030118)

  • short sperm flagellum(MP:0009239)

  • sinus inflammation(MP:0001872)

  • sneezing(MP:0031089)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV PolyA signal, mouse TL1 genomic DNA
    Research application
    Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
    The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
    In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
    The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
    DepositorMitsutoshi Setou (Mitsubishi Kasei Institute of Life Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Wu HY, Rong Y, Bansal PK, Wei P, Guo H, Morgan JI.
    TTLL1 and TTLL4 polyglutamylases are required for the neurodegenerative phenotypes in pcd mice.
    PLoS Genet 18(4) e1010144(2022) 35404950