Strain Data Sheet

RBRC03418

Strain Information

Image
BRC No.RBRC03418
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Apoe<tm(APOE4)Sfu>/SfuRbrc
Former Common nameApoE4 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shinobu Fujita, Mitsubishi Kagaku Institute of Life Sciences. 129 derived ES cells were wsed. The mice were crossed to C57BL/6.
Strain descriptionHuman ApoE4 knock-in mice. The mouse ApoE sequences including part of exon 2, entire exon 3 and most of exon 4 was replaced by the human APOE4 and a floxed neo casette.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NJcl]
References
Altered cholesterol metabolism in human apolipoprotein E4 knock-in mice.
Hamanaka H, Katoh-Fukui Y, Suzuki K, Kobayashi M, Suzuki R, Motegi Y, Nakahara Y, Takeshita A, Kawai M, Ishiguro K, Yokoyama M, Fujita S C
Hum. Mol. Genet., 9(3):353-361 (2000). 10655544

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
APOE4 apolipoprotein E4 (human)7APOE4
Apoe
MGI:88057		apolipoprotein E7Apoe<tm> targeted mutation
  • age related macular degeneration 1(MedGEN)

  • Alzheimer disease 2(DisGeNET, MedGEN)

  • Alzheimer disease 3(MedGEN)
    • more 4 Diseases
  • Alzheimer disease 4(MedGEN)

  • hyperlipoproteinemia type 3(DisGeNET, MedGEN)

  • lipoprotein glomerulopathy(DisGeNET, MedGEN)

  • sea-blue histiocyte syndrome(MedGEN)
    • loxP phage P1 loxP7loxP
    loxP phage P1 loxP7loxP
    neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal brain morphology(MP:0002152)

  • abnormal circulating cholesterol level(MP:0000180)

  • abnormal phospholipid level(MP:0004777)

  • abnormal plasma membrane morphology(MP:0003950)

  • abnormal response to CNS ischemic injury(MP:0003075)
    • more 4 phenotypes
  • decreased brain cholesterol level(MP:0010161)

  • increased circulating LDL cholesterol level(MP:0000182)

  • increased circulating VLDL cholesterol level(MP:0005145)

  • increased circulating cholesterol level(MP:0005178)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman APOE4 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Apoe genomic DNA
    Research applicationCardiovascular Research
    Cre/loxP system
    Hematological Research
    Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
    The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
    In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
    The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
    DepositorShinobu Fujita (Mitsubishi Kasei Institute of Life Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Takebayashi Y, Yamazaki Y, Yamada H, Yazawa K, Nakamori M, Kurashige T, Morino H, Takahashi T, Sotomaru Y, Maruyama H.
    Apolipoprotein E genotype-dependent accumulation of amyloid β in APP-knock-in mouse model of Alzheimer's disease.
    Biochem Biophys Res Commun 683 149106(2023) 37857162
    Yoshiko Takebayashi, Yu Yamazaki, Hidetada Yamada, Kyosuke Yazawa, Masahiro Nakamori, Takashi Kurashige, Hiroyuki Morino, Tetsuya Takahashi, Yusuke Sotomaru, Hirofumi Maruyama
    Apolipoprotein E genotype-dependent accumulation of amyloid β in APP-knock-in mouse model of Alzheimer's disease
    Biochemical and Biophysical Research Communications (2023)
    Mok KK, Yeung SH, Cheng GW, Ma IW, Lee RH, Herrup K, Tse KH.
    Apolipoprotein E ε4 disrupts oligodendrocyte differentiation by interfering with astrocyte-derived lipid transport.
    J Neurochem 165(1) 55-75(2023) 36549843
    Honda K, Saito Y, Saito H, Toyoda M, Abe R, Saito T, Saido TC, Michikawa M, Taru H, Sobu Y, Hata S, Nakaya T, Suzuki T.
    Accumulation of amyloid-β in the brain of mouse models of Alzheimer's disease is modified by altered gene expression in the presence of human apoE isoforms during aging.
    Neurobiol Aging 123 63-74(2023) 36638682
    Cheng GW, Mok KK, Yeung SH, Kofler J, Herrup K, Tse KH.
    Apolipoprotein E ε4 Mediates Myelin Breakdown by Targeting Oligodendrocytes in Sporadic Alzheimer Disease.
    J Neuropathol Exp Neurol 81(9) 717-730(2022) 35779013