Strain Data Sheet

RBRC03419

Strain Information

Image
BRC No.RBRC03419
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Apoe<tm2(APOE)Sfu>/43SfuRbrc
Former Common nameApoE2-1 KO
H-2 Haplotype
ES Cell lineE14TG2aIV [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Shinobu Fujita, Mitsubishi Kagaku Institute of Life Sciences. E14TG2aIV ES cells (129P2/OlaHsd derived) were used. The mice were crossed to C57BL/6.
Strain descriptionHuman ApoE2 knock-in mice line 1 (clone #43 derived). The mouse ApoE sequences including part of exon 2, entire exon 3 and most of exon 4 was replaced by the human APOE2 and a floxed neo casette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References
Altered cholesterol metabolism in human apolipoprotein E4 knock-in mice.
Hamanaka H, Katoh-Fukui Y, Suzuki K, Kobayashi M, Suzuki R, Motegi Y, Nakahara Y, Takeshita A, Kawai M, Ishiguro K, Yokoyama M, Fujita S C
Hum. Mol. Genet., 9(3):353-361 (2000). 10655544

Health Report

Examination Date / Room / Rack2024/12/09Room:3-BRack:ASentinel mouse program
2024/10/07Room:3-BRack:ASentinel mouse program
2024/07/08Room:3-BRack:AAdditional tests
2024/04/08Room:3-BRack:ASentinel mouse program
2024/01/09Room:3-BRack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
APOE2 apolipoprotein E2 (human)7APOE2
Apoe
MGI:88057		apolipoprotein E7Apoe<tm2(APOE)Sfu>
MGI:3613513		targeted mutation 2, Shinobu C Fujita
  • age related macular degeneration 1(MedGEN)

  • Alzheimer disease 2(DisGeNET, MedGEN)

  • Alzheimer disease 3(MedGEN)
    • more 4 Diseases
  • Alzheimer disease 4(MedGEN)

  • hyperlipoproteinemia type 3(DisGeNET, MedGEN)

  • lipoprotein glomerulopathy(DisGeNET, MedGEN)

  • sea-blue histiocyte syndrome(MedGEN)
    • loxP phage P1 loxP7loxP
    loxP phage P1 loxP7loxP
    neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal response to CNS ischemic injury(MP:0003075)

  • decreased circulating cholesterol level(MP:0005179)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman APOE2 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Apoe genomic DNA
    Research applicationCardiovascular Research
    Cre/loxP system
    Hematological Research
    Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
    The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
    In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
    The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
    DepositorShinobu Fujita (Mitsubishi Kasei Institute of Life Sciences)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data