Strain Data Sheet

RBRC03456

Strain Information

Image
BRC No.RBRC03456
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6N.129S2-Lxn<tm1Yari>/YariRbrc
Former Common nameLxn KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Yasuyoshi Arimatsu, Mitsubishi Kagaku Institute of Life Sciences. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6N.
Strain descriptionLxn (latexin) gene knockout mice. Exon 1 of the Lxn gene was replaced with a neomycin resistant cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References
Reduced pain sensitivity in mice lacking latexin, an inhibitor of metallocarboxypeptidases.
Jin M, Ishida M, Katoh-Fukui Y, Tsuchiya R, Higashinakagawa T, Ikegami S, Arimatsu Y
Brain Res., 1075(1):117-21 (2006). 16469302

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Lxn
MGI:107633		latexin3Lxn<tm1Yari>
MGI:3622076		targeted mutation 1, Yasuyoshi Arimatsu
neo neomycin resistance gene (E. coli)3 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • increased thermal nociceptive threshold(MP:0001973)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene,　mouse Lxn genomic DNA
    Research application
    Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
    The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
    In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
    The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
    DepositorYasuyoshi Arimatsu (Mitsubishi Kasei Institute of Life Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Xuchen Sun, Xuanming Chen, Yuanting Ni, Xiuzhen Li, Jiaqi Song, Jingzhu Wang, Shaohua Xu, Wei Shu, Ming Chen
    Latexin (LXN) enhances tumor immune surveillance in mice by inhibiting Treg cells through the macrophage exosome pathway
    International Journal of Biological Macromolecules 289 138822(2025) 39694381
    He G, Ni Y, Hua R, Wan H, Tan Y, Chen Q, Xu S, Yang Y, Zhang L, Shu W, Huang KB, Mo Y, Liang H, Chen M.
    Latexin deficiency limits foam cell formation and ameliorates atherosclerosis by promoting macrophage phenotype differentiation.
    Cell Death Dis 15(10) 754(2024) 39424784
    He G, Kan S, Xu S, Sun X, Li R, Shu W, Chen M.
    LXN deficiency regulates cytoskeleton remodelling by promoting proteolytic cleavage of Filamin A in vascular endothelial cells.
    J Cell Mol Med 25(14) 6815-6827(2021) 34085389