Strain Information | |
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Image | |
BRC No. | RBRC03460 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S2-Sgms1<tm1Kenw> |
Former Common name | GOR2KO |
H-2 Haplotype | |
ES Cell line | D3 [129S2/SvPas] |
Background strain | |
Appearance | |
Strain development | Developed by Ken Watanabe, Research Institute, National Center for Geriatrics and Gerontology. Backcross to C57BL/6 for 3 generations, then, maintained by sibling mating. |
Strain description | |
Colony maintenance | |
References | Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice. Yano M, Watanabe K, Yamamoto T, Ikeda K, Senokuchi T, Lu M, Kadomatsu T, Tsukano H, Ikawa M, Okabe M, Yamaoka S, Okazaki T, Umehara H, Gotoh T, Song W J, Node K, Taguchi R, Yamagata K, Oike Y J. Biol. Chem., 286 (5) 3992-4002 (2011). 21115496 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Sgms1 | sphingomyelin synthase 1 | 19 | Sgms1 | targeted mutation 1, Ken Watanabe | |||
loxP | phage P1 loxP | 19 | loxP | ||||
loxP | phage P1 loxP | 19 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 19 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 5 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | bacteriophage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sgms1 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Ken Watanabe (National Center for Geriatrics and Gerontology) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |