Strain Data Sheet

RBRC03461

Strain Information

Image
BRC No.RBRC03461
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Sgms2<tm1Kenw>
Former Common nameGOR1-53
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Ken Watanabe, Research Institute, National Center for Geriatrics and Gerontology.
Strain description
Colony maintenance
References
Dynamic modification of sphingomyelin in lipid microdomains controls development of obesity, fatty liver, and type 2 diabetes.
Mitsutake S, Zama K, Yokota H, Yoshida T, Tanaka M, Mitsui M, Ikawa M, Okabe M, Tanaka Y, Yamashita T, Takemoto H, Okazaki T, Watanabe K, Igarashi Y
J. Biol. Chem., 286, 28544-28555 (2011). 21669879

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sgms2
MGI:1921692		sphingomyelin synthase 23Sgms2<tm1Kenw>
MGI:5292035		targeted mutation 1, Ken Watanabe
  • calvarial doughnut lesions-bone fragility syndrome(MedGEN)
    • lacZ beta-galactosidase (E. coli)3
    loxP phage P1 loxP3loxP
    loxP phage P1 loxP3loxP
    neo neomycin resistance gene (E. coli)3 mouse phosphoglycerate kinase promoter (PGK promoter)
    nls SV40 large T antigen nuclear localization signal (NLS)3nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cell physiology(MP:0005621)

  • abnormal circulating glucose level(MP:0000188)

  • decreased circulating triglyceride level(MP:0002644)

  • decreased epididymal fat pad weight(MP:0009289)

  • decreased susceptibility to diet-induced obesity(MP:0005659)
    • more 4 phenotypes
  • decreased susceptibility to hepatic steatosis(MP:0002310)

  • improved glucose tolerance(MP:0005292)

  • increased circulating free fatty acids level(MP:0001554)

  • increased insulin sensitivity(MP:0002891)
    • Detailed phenotype data

    Ordering Information

    Donor DNASV40 large T antigen nuclear localization signal (NLS), E. coli lacZ, Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sgms2 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorKen Watanabe (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data