Strain Data Sheet

RBRC03512

Strain Information

Image
BRC No.RBRC03512
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rspo2<tm1Hoka>/Rbrc
Former Common nameR-spondin2(lacZ) (Acc.No. CDB0467K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
R-spondin2 expression in the apical ectodermal ridge is essential for outgrowth and patterning in mouse limb development.
Aoki M, Kiyonari H, Nakamura H, Okamoto H
Dev. Growth. Differ., 50, 85-95 (2008). 18067586

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site15Frt
Frt yeast FRT (flippase recombination target) site15Frt
HBB2 rabbit beta-globin polyA15HBB2
Rspo2
MGI:1922667		R-spondin 215Rspo2<tm1Hoka>
MGI:3783769		targeted mutation 1, Hitoshi Okamoto
  • humerofemoral hypoplasia with radiotibial ray deficiency(MedGEN)

  • tetraamelia syndrome 2(MedGEN)
    • lacZ beta-galactosidase (E. coli)15
    lox71 mutant lox sequence15lox71
    loxP phage P1 loxP15loxP
    neo neomycin resistance gene (E. coli)15 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal apical ectodermal ridge morphology(MP:0001676)

  • abnormal autopod morphology(MP:0000572)

  • abnormal digit morphology(MP:0002110)

  • abnormal forelimb morphology(MP:0000550)

  • abnormal forelimb zeugopod morphology(MP:0003855)
    • more 5 phenotypes
  • abnormal hindlimb morphology(MP:0000556)

  • abnormal limb development(MP:0006279)

  • abnormal phalanx morphology(MP:0005306)

  • absent fibula(MP:0005430)

  • perinatal lethality, complete penetrance(MP:0011089)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 lox71, E. coli LacZ, poly A, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase (Pgk) promoter, E. coli neo, phage P1 loxP, rabbit beta-globin polyA, mouse Rspo2 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Hitoshi Okamoto (hitoshi@brain.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0467K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Growth Differ. 2008 50(2):85-95.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data