Strain Data Sheet

RBRC03515

Strain Information

Image
BRC No.RBRC03515
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aire<tm2Mmat>/Rbrc
Former Common nameAire(GFP) (Acc.No. CDB0479K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology and Mitsuru Matsumoto, Institute for Enzyme Research, University of Tokushima. TT2 ES cells were used.
Strain descriptionAire GFP knock-in mice. A GFP was inserted in the Aire gene locus between exon 1 and 2.
Colony maintenance
References
Aire controls the differentiation program of thymic epithelial cells in the medulla for the establishment of self-tolerance.
Yano M, Kuroda N, Han H, Meguro-Horike M, Nishikawa Y, Kiyonari H, Maemura K, Yanagawa Y, Obata K, Takahashi S, Ikawa T, Satoh R, Kawamoto H, Mouri Y, Matsumoto M
J. Exp. Med., 205, 2827-2838 (2008). 19015306

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aire
MGI:1338803		autoimmune regulator10Aire<tm2Mmat>
MGI:3818933		targeted mutation 2, Mitsuru Matsumoto
  • autoimmune polyendocrine syndrome type 1(DisGeNET, MedGEN)
    • GFP Green Fluorescent Protein (Aequorea victoria)10
    loxP phage P1 loxP10loxP
    loxP phage P1 loxP10loxP
    neo neomycin resistance gene (E. coli)10 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal thymus morphology(MP:0000703)

  • abnormal thymus physiology(MP:0003763)

  • absent Hassall's corpuscle(MP:0009540)

  • autoimmune response(MP:0001844)

  • immune system phenotype(MP:0005387)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neo, jellyfish GFP cDNA, mouse Aire genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Mitsuru Matsumoto (mitsuru@ier.tokushima-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0479K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Exp Med. 2008 205(12):2827-38.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ishikawa T, Horie K, Takakura Y, Ohki H, Maruyama Y, Hayama M, Miyauchi M, Miyao T, Hagiwara N, Kobayashi TJ, Akiyama N, Akiyama T.
    T-cell receptor repertoire analysis of CD4-positive T cells from blood and an affected organ in an autoimmune mouse model.
    Genes Cells 28(12) 929-941(2023) 37909727