Strain Information | |
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Image | |
BRC No. | RBRC03519 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6D2;129P2-Cthrc1<tm1Hssk>/Rbrc |
Former Common name | Cthrc1(lacZ) (Acc.No. CDB0502K) |
H-2 Haplotype | |
ES Cell line | E14TG2a [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Hiroshi Sasaki, RIKEN Center for Developmental Biology. A lacZ and PGK-neo cassette was transferred into E14TG2a ES cells to replace the exon 1 of Cthrc1 gene. |
Strain description | Cthrc1 gene knockout mice. Cthrc1 is a Wnt signaling component that selectively activates the PCP pathway. |
Colony maintenance | |
References | Cthrc1 selectively activates the planar cell polarity pathway of Wnt signaling by stabilizing the Wnt-receptor complex. Yamamoto S, Nishimura O, Misaki K, Nishita M, Minami Y, Yonemura S, Tarui H, Sasaki H Dev. Cell., 15, 23-36 (2008). 18606138 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Cthrc1 MGI:1915838 | collagen triple helix repeat containing 1 | 15 | Cthrc1<tm1Hssk> MGI:3815067 | targeted mutation 1, Hiroshi Ssasaki | |||
Frt | yeast FRT (flippase recombination target) site | 15 | Frt | ||||
HBB2 | rabbit beta-globin 3' end | 15 | HBB2 | ||||
lacZ | beta-galactosidase (E. coli) | 15 | |||||
lox71 | mutant lox sequence | 15 | lox71 | ||||
loxP | phage P1 loxP | 15 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 15 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 lox71 site and loxP site, E. coli lacZ, rabbit beta-globin 3' end, yeast FRT (flipase recombination target) sites, mouse PGK promoter and PGK 3' end, E. coli neo, mouse Cthrc1 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0502K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Cell. 2008 15(1):23-36.) (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Li J, Wang Y, Ma M, Jiang S, Zhang X, Zhang Y, Yang X, Xu C, Tian G, Li Q, Wang Y, Zhu L, Nie H, Feng M, Xia Q, Gu J, Xu Q, Zhang Z. Autocrine CTHRC1 activates hepatic stellate cells and promotes liver fibrosis by activating TGF-β signaling. EBioMedicine 40 43-55(2019) 30639416 |