Strain Data Sheet

RBRC03519

Strain Information

Image
BRC No.RBRC03519
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6D2;129P2-Cthrc1<tm1Hssk>/Rbrc
Former Common nameCthrc1(lacZ) (Acc.No. CDB0502K)
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Sasaki, RIKEN Center for Developmental Biology. A lacZ and PGK-neo cassette was transferred into E14TG2a ES cells to replace the exon 1 of Cthrc1 gene.
Strain descriptionCthrc1 gene knockout mice. Cthrc1 is a Wnt signaling component that selectively activates the PCP pathway.
Colony maintenance
References
Cthrc1 selectively activates the planar cell polarity pathway of Wnt signaling by stabilizing the Wnt-receptor complex.
Yamamoto S, Nishimura O, Misaki K, Nishita M, Minami Y, Yonemura S, Tarui H, Sasaki H
Dev. Cell., 15, 23-36 (2008). 18606138

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cthrc1
MGI:1915838
collagen triple helix repeat containing 115Cthrc1<tm1Hssk>
MGI:3815067
targeted mutation 1, Hiroshi Ssasaki
  • Barrett esophagus(MedGEN)
  • Frt yeast FRT (flippase recombination target) site15Frt
    HBB2 rabbit beta-globin 3' end15HBB2
    lacZ beta-galactosidase (E. coli)15
    lox71 mutant lox sequence15lox71
    loxP phage P1 loxP15loxP
    neo neomycin resistance gene (E. coli)15

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 lox71 site and loxP site, E. coli lacZ, rabbit beta-globin 3' end, yeast FRT (flipase recombination target) sites, mouse PGK promoter and PGK 3' end, E. coli neo, mouse Cthrc1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0502K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Cell. 2008 15(1):23-36.) (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Li J, Wang Y, Ma M, Jiang S, Zhang X, Zhang Y, Yang X, Xu C, Tian G, Li Q, Wang Y, Zhu L, Nie H, Feng M, Xia Q, Gu J, Xu Q, Zhang Z.
    Autocrine CTHRC1 activates hepatic stellate cells and promotes liver fibrosis by activating TGF-β signaling.
    EBioMedicine 40 43-55(2019) 30639416