Strain Data Sheet

RBRC03558

Strain Information

Image
BRC No.RBRC03558
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129.Cg-Pax7<tm1Pgr>
Former Common namePax129, 129-Pax7 KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Peter Gruss, Max Planck Institute for Biophysical Chemistry. The mutant mice were backcrossed 129 over 20 times by Naohiro Hashimoto, National Center for Geriatrics and Gerontology.
Strain descriptionPax7 gene knockout mice. The first exon of the paired box was replaced with a neo cassette (RBRC03558) and lacZ-neo cassette (RBRC03559), respectively. In the C57BL/6 background, most homozygous mutant mice die within 3 weeks after birth (survival rate is below 10%). On the other hand, in the 129 background (RBRC03558 and RBRC03559), approximately 50% of mutant mice die within 3 weeks after birth (details unknown). Heterozygous mutant mice for lacZ repoter Pax7<tm2Pgr> (RBRC03559) show negative X-gal staining in the muscle satellite cells.
Colony maintenanceHeterozygote x Wild-type [or Crossing to 129<+Ter>/SvJcl]
References
Dysgenesis of cephalic neural crest derivatives in Pax7-/- mutant mice.
Mansouri A, Stoykova A, Torres M, Gruss P
Development, 122, 831-838 (1996). 8631261

Pax7 is required for the specification of myogenic satellite cells.
Seale P, Sabourin L A, Girgis-Gabardo A, Mansouri A, Gruss P, Rudnicki M A
Cell, 102, 777-786 (2000). 11030621

Health Report

Examination Date / Room / Rack2023/11/27Room:4-CRack:KSentinel mouse program
2023/08/28Room:4-CRack:KSentinel mouse program
2023/05/29Room:4-CRack:KSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pax7
MGI:97491		paired box 74Pax7<tm1Pgr>
MGI:1857831		targeted mutation 1, Peter Gruss
  • alveolar rhabdomyosarcoma(DisGeNET, MedGEN)

  • myopathy, congenital, progressive, with scoliosis(MedGEN)
    • neo neomycin resistance gene (Klebsiella pneumoniae)4 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal muscle regeneration(MP:0000750)

  • abnormal nasal capsule morphology(MP:0004726)

  • abnormal serous gland morphology(MP:0008052)

  • cellular phenotype(MP:0005384)

  • decreased skeletal muscle fiber diameter(MP:0009402)
    • more 11 phenotypes
  • digestive/alimentary phenotype(MP:0005381)

  • embryo phenotype(MP:0005380)

  • muscle phenotype(MP:0005369)

  • muscle weakness(MP:0000747)

  • nervous system phenotype(MP:0003631)

  • pointed snout(MP:0000448)

  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • premature death(MP:0002083)

  • short maxilla(MP:0000097)

  • thin diaphragm muscle(MP:0000761)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), Klebsiella pneumoniae neomycin resistance gene, bovine growth hormone polyadenylation signal, mouse Myod1 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Pax7tm1Pgr allele (Peter Gruss, Max Planck Institute for Biophysical Chemistry). (https://www.oist.jp/peter-gruss)
    DepositorNaohiro Hashimoto (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. MATERIAL TRANSFER AGREEMENT (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data