Strain Data Sheet

RBRC03705

Strain Information

Image
BRC No.RBRC03705
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(Nes-cre)52Utr/Rbrc
Former Common nameNestin-NLSCre
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Laboratory Animal Resource Center, Tsukuba University and RIKEN BioResource Center in 2009. C57BL/6N background.
Strain descriptionCre-driver transgenic mice. A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse nestin promoter fragment derived from the BAC library (BAC clone RP24-64E17)was microinjected into C57BL/6NCrlCrlj fertilized eggs. Tissue-specific expression of Cre recombinase has not been examined yet. Users are encouraged to test the tissue-specificity and feed back the data for the research community.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown).
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteUNFrt
cre Phage P1 Cre recombinaseUNcre
loxP phage P1 loxPUNloxP
nls SV40 large T antigen nuclear localization signal (NLS)UNnls mouse Nestin promoter (neuronal-specific promoter)
SV40 polyAUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACre recombinase [P1_ap003, Bacteriophage genomic DNA (class 1)], FRT sequence [yeast genomic DNA (class 1)], Nestin [mouse genomic DNA (neuronal-specific promoter)(class 1)], Phage P1 loxP derived from pBACe3.6 vector, BAC clone RP23-141A13, SV40 poly A, SV40 Large T antigen nuclear localization signal (NLS)
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data