Strain Data Sheet

RBRC03709

Strain Information

Image
BRC No.RBRC03709
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Disc1<tm1Kozo>
Former Common nameDISC1 (delta 2-3), DISC1 KO, Disc1(-) (Acc.No. CDB0583K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Keisuke Kuroda, Kozo Kaibuchi, Nagoya University, Graduate School of Medicine in 2008. A loxP/PGK-Neo-pA/loxP cassette was transferred into TT2 ES cells to replace most of exon 2 and all of exon 3 of Disc1 gene. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionDisc1 knockout mice. Most of exon 2 and all of exon 3 of Disc1 gene were replaced with a loxP/PGK-Neo-pA/loxP cassette. Homozygous mutant mice are viable and fertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
Behavioral alterations associated with targeted disruption of exons 2 and 3 of the Disc1 gene in the mouse.
Kuroda K, Yamada S, Tanaka M, Iizuka M, Yano H, Mori D, Tsuboi D, Nishioka T, Namba T, Iizuka Y, Kubota S, Nagai T, Ibi D, Wang R, Enomoto A, Isotani-Sakakibara M, Asai N, Kimura K, Kiyonari H, Abe T, Mizoguchi A, Sokabe M, Takahashi M, Yamada K, Kaibuchi K
Hum. Mol. Genet., 20, 4666-4683 (2011). 21903668

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Disc1
MGI:2447658
disrupted in schizophrenia 18Disc1<tm1Kozo>
MGI:5296951
targeted mutation 1, Kozo Kaibuchi
  • schizophrenia 9(MedGEN)
  • loxP phage P1 loxP8loxP
    loxP phage P1 loxP8loxP
    neo neomycin resistance gene (E. coli)8 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal impulsive behavior control(MP:0010698)

  • abnormal long-term potentiation(MP:0002207)

  • abnormal response to novel object(MP:0003461)

  • abnormal social investigation(MP:0001360)

  • abnormal synaptic depression(MP:0002915)
  • more 9 phenotypes
  • behavior/neurological phenotype(MP:0005386)

  • decreased anxiety-related response(MP:0001364)

  • decreased prepulse inhibition(MP:0009142)

  • decreased serotonin level(MP:0010070)

  • enhanced contextual conditioning behavior(MP:0009453)

  • hyperactivity(MP:0001399)

  • induced hyperactivity(MP:0008911)

  • nervous system phenotype(MP:0003631)

  • reduced long-term potentiation(MP:0001473)
  • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neomycin resistance gene, Mouse phosphoglycerate kinase Poly A signal, mouse Disc1 genome DNA
    Research applicationCre/loxP system
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 20, 4666-4683 (2011). (1) The RECIPIENT shall obtain a prior agreement from Dr. Kouzo Kaibuchi (kaibuchi@med.nagoya-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0583K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (未定) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Obi-Nagata K, Suzuki N, Miyake R, MacDonald ML, Fish KN, Ozawa K, Nagahama K, Okimura T, Tanaka S, Kano M, Fukazawa Y, Sweet RA, Hayashi-Takagi A.
    Distorted neurocomputation by a small number of extra-large spines in psychiatric disorders.
    Sci Adv 9(23) eade5973(2023) 37294752
    Onishi T, Sakamoto H, Namiki S, Hirose K.
    The Altered Supramolecular Structure of Dopamine D2 Receptors in Disc1-deficient Mice.
    Sci Rep 8(1) 1692(2018) 29374282