Strain Information | |
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Image | |
BRC No. | RBRC03709 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.B6CB-Disc1<tm1Kozo> |
Former Common name | DISC1 (delta 2-3), DISC1 KO, Disc1(-) (Acc.No. CDB0583K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6 |
Appearance | |
Strain development | Developed by Keisuke Kuroda, Kozo Kaibuchi, Nagoya University, Graduate School of Medicine in 2008. A loxP/PGK-Neo-pA/loxP cassette was transferred into TT2 ES cells to replace most of exon 2 and all of exon 3 of Disc1 gene. The mutant mice were backcrossed to C57BL/6J. |
Strain description | Disc1 knockout mice. Most of exon 2 and all of exon 3 of Disc1 gene were replaced with a loxP/PGK-Neo-pA/loxP cassette. Homozygous mutant mice are viable and fertile. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJmsSlc] |
References | Behavioral alterations associated with targeted disruption of exons 2 and 3 of the Disc1 gene in the mouse. Kuroda K, Yamada S, Tanaka M, Iizuka M, Yano H, Mori D, Tsuboi D, Nishioka T, Namba T, Iizuka Y, Kubota S, Nagai T, Ibi D, Wang R, Enomoto A, Isotani-Sakakibara M, Asai N, Kimura K, Kiyonari H, Abe T, Mizoguchi A, Sokabe M, Takahashi M, Yamada K, Kaibuchi K Hum. Mol. Genet., 20, 4666-4683 (2011). 21903668 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Disc1 MGI:2447658 | disrupted in schizophrenia 1 | 8 | Disc1<tm1Kozo> MGI:5296951 | targeted mutation 1, Kozo Kaibuchi | |||
loxP | phage P1 loxP | 8 | loxP | ||||
loxP | phage P1 loxP | 8 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 8 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 9 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neomycin resistance gene, Mouse phosphoglycerate kinase Poly A signal, mouse Disc1 genome DNA |
Research application | Cre/loxP system Mouse Models for Human Disease |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 20, 4666-4683 (2011). (1) The RECIPIENT shall obtain a prior agreement from Dr. Kouzo Kaibuchi (kaibuchi@med.nagoya-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0583K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (未定) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Obi-Nagata K, Suzuki N, Miyake R, MacDonald ML, Fish KN, Ozawa K, Nagahama K, Okimura T, Tanaka S, Kano M, Fukazawa Y, Sweet RA, Hayashi-Takagi A. Distorted neurocomputation by a small number of extra-large spines in psychiatric disorders. Sci Adv 9(23) eade5973(2023) 37294752 |
Onishi T, Sakamoto H, Namiki S, Hirose K. The Altered Supramolecular Structure of Dopamine D2 Receptors in Disc1-deficient Mice. Sci Rep 8(1) 1692(2018) 29374282 |