Strain Data Sheet

RBRC03712

Strain Information

Image
BRC No.RBRC03712
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129P2-Dgcr8<Gt(XG058)Byg>
Former Common nameDGCR8KO/XG058
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Takashi Iwamoto, Chubu University in 2007.
Strain descriptionDGCR8 is a key molecule of biogenesis of microRNA. DGCR8-/- mice are embryonic lethal and DGCR8-/+ mice look healthy but could be models of human 22q11.2 deletion syndrome including schizophrenia.
Colony maintenance
References
DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal.
Wang Y, Medvid R, Melton C, Jaenisch R, Blelloch R
Nat. Genet., 39, 380-385 (2007). 17259983

Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model.
Stark K L, Xu B, Bagchi A, Lai W S, Liu H, Hsu R, Wan X, Pavlidis P, Mills A A, Karayiorgou M, Gogos J A
Nat. Genet., 40, 751-760 (2008). 18469815

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal brain interneuron morphology(MP:0004101)

  • abnormal nest building behavior(MP:0001447)

  • abnormal neuronal migration(MP:0006009)

  • abnormal neuronal precursor proliferation(MP:0004948)

  • abnormal spatial working memory(MP:0008428)
    • more 6 phenotypes
  • behavior/neurological phenotype(MP:0005386)

  • behavioral despair(MP:0002573)

  • decreased dentate gyrus size(MP:0012460)

  • embryonic lethality between implantation and somite formation, complete penetrance(MP:0011096)

  • impaired spatial learning(MP:0012307)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE coli Neomycin resistance gene, E coli beta-galactosidase, mouse EN2 gene intron, mouse Splice acceptor of EN2 gene exon2
    Research application
    Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. All data got with this BIOLOGICAL RESOURCE must be published as a joint result with the DEPOSITOR.
    DepositorTakashi Iwamoto (Chubu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data