Strain Information | |
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Image | |
BRC No. | RBRC03733 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129S4-Dnmt3b<tm5.1Enl> |
Former Common name | Dnmt3b<tm5.1Enl>; Dnmt3b conditional KO, Dnmt3b-2lox |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Developed by Masaki Okano and En Li, Massachusetts General Hospital in 2001. J1 ES cells were used to generate the mutant mice. The mutant mice were crossed to C57BL/6. |
Strain description | Dnmt3b floxed mice. Exon 19 of the Enmt3b was flanked by loxP sites. Conditional Dnmt3a deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice are viable and fertile. Dnmt3a knockout mice (RBRC03730), Dnmt3a floxed mice (RBRC03731), Dnmt3b knockout mice (RBRC03732). |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Inactivation of Dnmt3b in mouse embryonic fibroblasts results in DNA hypomethylation, chromosomal instability, and spontaneous immortalization. Dodge J E, Okano M, Dick F, Tsujimoto N, Chen T, Wang S, Ueda Y, Dyson N, Li E J. Biol. Chem., 280, 17986-17991 (2005). 15757890 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Dnmt3b MGI:1261819 | DNA methyltransferase 3B | 2 | Dnmt3b<tm5.1Enl> | targeted mutation 5.1, En Li | |||
loxP | phage P1 loxP | 2 | loxP | ||||
loxP | phage P1 loxP | 2 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 LoxP sites, mouse Dnmt3b genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | A. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA (phsmta@partners.org). B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature J Biol Chem. 2005 280:17986-91 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient. |
Depositor | Masaki Okano (RIKEN CDB/ MGH) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Yao S, Prates K, Freydenzon A, Assante G, McRae AF, Morris MJ, Youngson NA. Liver-specific deletion of de novo DNA methyltransferases protects against glucose intolerance in high-fat diet-fed male mice. FASEB J 38(10) e23690(2024) 38795327 |
Matsuzaki H, Okamura E, Takahashi T, Ushiki A, Nakamura T, Nakano T, Hata K, Fukamizu A, Tanimoto K. De novo DNA methylation through the 5'-segment of the H19 ICR maintains its imprint during early embryogenesis. Development 142(22) 3833-44(2015) 26417043 |