Strain Data Sheet

RBRC03743

Strain Information

Image
BRC No.RBRC03743
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb
Former Common nameB6D2F1-Tg(CAG/su9-DsRed2,Acr3-EGFP), RBGS002Osb (Nov. 2011), RBGS-002
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. This transgenic line was generated by coinjected with Acr3-EGFP and CAG/su9-DsRed2 transgene constructs into fertilized eggs. C57BL/6 and DBA/2 mixed background.
Strain descriptionTrangenic mice expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2. The dual fluorescent sperm show normal fertilizing ability and can be observed through uterine and oviductal walls under excitation light. Acrosome reacted sperm and acrosome intact sperm are easily distinguishable from each other by red fluorescence. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG/su9-DsRed2, in which a RFP(DsRed2) is connected to the CAG promoter with a mitochondrial import signal sequence of Atp5g1(su9).
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6D2F1]
References
Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo.
Hasuwa H, Muro Y, Ikawa M, Kato N, Tsujimoto Y, Okabe M
Exp. Anim., 59:105-107(2010). 20224175

Health Report

Examination Date / Room / Rack2025/01/07Room:3-BRack:CSentinel mouse program
2024/10/07Room:3-BRack:CSentinel mouse program
2024/07/08Room:3-BRack:CSentinel mouse program
2024/04/08Room:3-BRack:CSentinel mouse program
2024/01/09Room:3-BRack:CSentinel mouse program
2023/10/10Room:3-BRack:CSentinel mouse program
2023/07/10Room:3-BRack:CSentinel mouse program
2023/04/10Room:3-BRack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
DsRed2 Red Fluorescent Protein (Discosoma sp.)UNDsRed2
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP acrosin promoter
su9 mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)]UNsu9 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)], Discosoma sp. DsRed2 cDNA, acrosin promoter, acrosin localization signal, jellyfish EGFP cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 59:105-107(2010). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: TBUSAMTA Nonprofit
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Wang Z, Wei H, Wu Z, Zhang X, Sun Y, Gao L, Zhang W, Su YQ, Zhang M.
    The oocyte cumulus complex regulates mouse sperm migration in the oviduct.
    Commun Biol 5(1) 1327(2022) 36463362
    Lück JC, Puchkov D, Ullrich F, Jentsch TJ.
    LRRC8/VRAC anion channels are required for late stages of spermatid development in mice.
    J Biol Chem 293(30) 11796-11808(2018) 29880644
    Hino T, Muro Y, Tamura-Nakano M, Okabe M, Tateno H, Yanagimachi R.
    The Behavior and Acrosomal Status of Mouse Spermatozoa In Vitro, and Within the Oviduct During Fertilization after Natural Mating.
    Biol Reprod 95(3) 50(2016) 27417908
    Luo SM, Ge ZJ, Wang ZW, Jiang ZZ, Wang ZB, Ouyang YC, Hou Y, Schatten H, Sun QY.
    Unique insights into maternal mitochondrial inheritance in mice.
    Proc Natl Acad Sci U S A 110(32) 13038-43(2013) 23878233