Strain Data Sheet

RBRC03799

update : 2025-02-20

Strain Information
Image
BRC No.	RBRC03799
Type	Targeted Mutation
Species	Mus musculus
Strain name	C.129X1(B6)-Sept4<tm1Ksh>
Former Common name	BALB/c-Sept4 KO
H-2 Haplotype
ES Cell line
Background strain	BALB/cCrSlc
Appearance
Strain development	Developed by Makoto Kinoshita, Kyoto University Graduate School of Medicine in 2005-2007.
Strain description	Homozygous mutant male are infertile.
Colony maintenance
References	Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa. Ihara M, Kinoshita A, Yamada S, Tanaka H, Tanigaki A, Kitano A, Goto M, Okubo K, Nishiyama H, Ogawa O, Takahashi C, Itohara S, Nishimune Y, Noda M, Kinoshita M Dev Cell., 8, 343-352 (2005). 15737930 Disruption of Sept6, a fusion partner gene of MLL, does not affect ontogeny, leukemogenesis induced by MLL-SEPT6, or phenotype induced by the loss of Sept4. Ono R, Ihara M, Nakajima H, Ozaki K, Kataoka-Fujiwara Y, Taki T, Nagata K, Inagaki M, Yoshida N, Kitamura T, Hayashi Y, Kinoshita M, Nosaka T Mol Cell Biol., 25, 10965-10978 (2005). 16314519 Sept4, a component of presynaptic scaffold and Lewy bodies, is required for the suppression of alpha-synuclein neurotoxicity. Ihara M, Yamasaki N, Hagiwara A, Tanigaki A, Kitano A, Hikawa R, Tomimoto H, Noda M, Takanashi M, Mori H, Hattori N, Miyakawa T, Kinoshita M Neuron, 53, 519-533 (2007). 17296554 Loss of Sept4 exacerbates liver fibrosis through the dysregulation of hepatic stellate cells. Iwaisako K, Hatano E, Taura K, Nakajima A, Tada M, Seo S, Tamaki N, Sato F, Ikai I, Uemoto S, Kinoshita M J. Hepatol., 49, 768-778 (2008). 18804890 Spatiotemporal association of DNAJB13 with the annulus during mouse sperm flagellum development. Guan J, Kinoshita M, Yuan L BMC Dev. Biol., 9, 23 (2009). 19298648

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Sept4 MGI:1270156	septin 4	11	Sept4	septin 4

Phenotype
Annotation by Mammalian phenotyhpe ontology	abnormal sperm fibrous sheath morphology(MP:0030590) abnormal sperm physiology(MP:0004543) abnormal sperm principal piece morphology(MP:0009836) absent sperm annulus(MP:0009835) asthenozoospermia(MP:0002675) more 2 phenotypes kinked sperm flagellum(MP:0009237) male infertility(MP:0001925)
Detailed phenotype data

Ordering Information
Donor DNA	mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sept4 genomic DNA
Research application
Specific Term and Conditions	The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell., 8, 343-352 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Depositor	Makoto Kinoshita (Nagoya University)
Strain Status	Frozen sperm
Strain Availability	Recovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP (Japanese) Genotyping protocol -PCR-

BRC mice in Publications

No Data