Strain Data Sheet
RBRC03801
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC03801 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6N-Tg(Vil1-cre)2Utr/Rbrc |
| Former Common name | Villin-NLSCre |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6NCrlCrlj |
| Appearance | |
| Strain development | Developed by Laboratory Animal Resource Center, Tsukuba University and RIKEN BioResource Center in 2009. C57BL/6N background. |
| Strain description | Cre-driver transgenic mice. A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse villin 1 promoter fragment (intestinal stem cells specific promoter region) derived from the BAC library was microinjected into C57BL/6NCrlCrlj fertilized eggs. Tissue-specific expression of Cre recombinase has not been examined yet. Users are encouraged to test the tissue-specificity and feed back the data for the research community. |
| Colony maintenance | Carrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown). |
| References | |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/10/07Room:3-ARack:FSentinel mouse program 2024/07/08Room:3-ARack:FSentinel mouse program 2024/04/08Room:3-ARack:FSentinel mouse program 2024/01/09Room:3-ARack:D and FSentinel mouse program 2023/10/10Room:3-ARack:DSentinel mouse program 2023/07/10Room:3-ARack:DSentinel mouse program 2023/04/10Room:3-ARack:DSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
| cre | Phage P1 Cre recombinase | UN | cre | ||||
| loxP | phage P1 loxP | UN | loxP | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | UN | nls | mouse Villin gene promoter (intestinal stem cells specific promoter region) | |||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | P1 phage Cre gene, yeast FRT (flipase recombination target) site, Mouse Villin gene promoter (intestinal stem cells specific promoter region), Phage P1 loxP derived from pBACe3.6 vector, Simian virus 40 Large T antigen nuclear localization signal (NLS), poly A |
| Research application | |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | RIKEN BRC (RIKEN BRC) |
| Strain Status |  Frozen embryos |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jul 2013 |
BRC mice in Publications |
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| No Data |