Strain Data Sheet

RBRC03899

Strain Information

Image
BRC No.RBRC03899
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Ephb1<tm1Cmn> Ephb2<tm1Paw> Ephb3<tm1Kln> EphB6<tm2Tom>
Former Common nameEphB1, EphB2, EphB3, EphB6 fourfold knockout mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toshimitsu Mastui, Kobe University Graduate School of Medicine. 129 and ICR mixed background.EphB1, EphB2, EphB3 KO: Mark Henkemeyer, University of Texas Southwestern Medical Center; EphB6 KO: Hiroshi Suzuki and Toshimitsu Mastui, Kobe University.
Strain description
Colony maintenance
References
Nuk controls pathfinding of commissural axons in the mammalian central nervous system.
Henkemeyer M, Orioli D, Henderson J T, Saxton T M, Roder J, Pawson T, Klein R
Cell, 86, 35-46, (1996). 8689685

Sek4 and Nuk receptors cooperate in guidance of commissural axons and in palate formation.
Orioli D, Henkemeyer M, Lemke G, Klein R, Pawson T
EMBO J., 15, 6035-6049 (1996). 8947026

Developmental expression of EphB6 in the thymus: lessons from EphB6 knockout mice.
Shimoyama M, Matsuoka H, Nagata A, Iwata N, Tamekane A, Okamura A, Gomyo H, Ito M, Jishage K, Kamada N, Suzuki H, Tetsuo Noda T, Matsui T
Biochem. Biophys. Res. Commun., 298, 87-94 (2002). 12379224

Ephrin-B2 and EphB1 mediate retinal axon divergence at the optic chiasm.
Williams S E, Mann F, Erskine L, Sakurai T, Wei S, Rossi D J, Gale N W, Holt C E, Mason C A, Henkemeyer M
Neuron., 39, 919-935 (2003). 12971893

Multiple EphB receptor tyrosine kinases shape dendritic spines in the hippocampus.
Henkemeyer M, Itkis O S, Ngo M, Hickmott P W, Ethell I M
J. Cell. Biol., 163, 1313-1326 (2003). 14691139

A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
Eur. J. Immunol., 42, 1562-1572 (2012). 22622783

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ephb3
MGI:104770		Eph receptor B316Ephb3<tm1Kln>
MGI:2149669		targeted mutation 1, Rudiger Klein
neo neomycin resistance gene (E. coli)16 mouse phosphoglycerate kinase promoter (PGK promoter)
Ephb2
MGI:99611		Eph receptor B24Ephb2<tm1Paw>
MGI:2149765		targeted mutation 1, Tony Pawson
  • bleeding disorder, platelet-type, 22(MedGEN)

  • prostate cancer/brain cancer susceptibility(MedGEN)
    • neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)
    Ephb6
    MGI:1096338    		Eph receptor B66Ephb6<tm2Tom>
    MGI:2389311    		targeted mutation 2, Toshimitsu Matsui
    loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)
    Ephb1
    MGI:1096337    		Eph receptor B19Ephb1<tm1Cm>
    MGI:2677305    		targeted mutation 1, Carol A Mason
    neo neomycin resistance gene (E. coli)9

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal Paneth cell morphology(MP:0004843)

  • abnormal anogenital distance(MP:0005189)

  • abnormal anterior commissure pars posterior morphology(MP:0010860)

  • abnormal axon guidance(MP:0002961)

  • abnormal axon morphology(MP:0005404)
    • more 31 phenotypes
  • abnormal axon pruning(MP:0008960)

  • abnormal digestive system physiology(MP:0001663)

  • abnormal endolymph physiology(MP:0004885)

  • abnormal eye morphology(MP:0002092)

  • abnormal inner ear vestibule morphology(MP:0000034)

  • abnormal large intestine crypts of Lieberkuhn morphology(MP:0004842)

  • abnormal optic nerve innervation(MP:0001332)

  • abnormal optic tract morphology(MP:0004267)

  • abnormal semicircular canal morphology(MP:0002428)

  • abnormal sensory neuron innervation pattern(MP:0000968)

  • abnormal small intestinal crypt cell proliferation(MP:0010157)

  • abnormal vestibular dark cell morphology(MP:0004775)

  • abnormal vestibular endolymph ionic homeostasis(MP:0006391)

  • abnormal vestibular endolymph physiology(MP:0006389)

  • abnormal vestibular system physiology(MP:0004742)

  • behavior/neurological phenotype(MP:0005386)

  • circling(MP:0001394)

  • cleft palate(MP:0000111)

  • delayed axon extension(MP:0002946)

  • digestive/alimentary phenotype(MP:0005381)

  • ectopic Paneth cells(MP:0014077)

  • head bobbing(MP:0001410)

  • hematopoietic system phenotype(MP:0005397)

  • hyperactivity(MP:0001399)

  • hypospadia(MP:0003124)

  • immune system phenotype(MP:0005387)

  • neoplasm(MP:0002006)

  • omphalocele(MP:0003052)

  • premature death(MP:0002083)

  • small endolymphatic duct(MP:0008066)

  • vision/eye phenotype(MP:0005391)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase (PGK) promoter, E. coli neo, Phage P1 loxP sites, mouse Ephb1 genomic DNA, mouse Ephb2 genomic DNA, mouse Ephb3 genomic DNA, mouse Ephb6 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Neurobiology Research
    Specific Term and Conditions1. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by use of the DEPOSITOR is requested. Henkemeyer et al., 1996 paper in Cell for EphB2/EphB3, Williams et al., 2003 paper in Neuron for EphB1. Shimoyama et al., 2002 paper in BBRC for EphB6, or Kawano et. al., 2012 paper in Eur. J. Immunol. for EphB1/EphB2/EphB3/EphB6 quadruple mutant mice.
    2. UT Southwestern would like to limit the use of the BIOLOGICAL RESOURCE to non-profit institutions, also limit the use to academic research purposes and for-profit institutions or use for commercial research purposes shall acquire a prior written consent from the Depositor, a recipient shall acquire a prior written consent form the Depositor prior to filing an application for a patent, or intellectual property or other right based on the results research using the BIOLOGICAL RESOURCE.
    DepositorDrs. Toshimitsu Matsui and Mark Henkemeyer (Kobe University/ University of Texas Southwestern Medical Center)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data