Strain Data Sheet

RBRC03914

Strain Information

Image
BRC No.RBRC03914
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Myh6-mCherry/LC3)1Acvm
Former Common namealpha MyHC-mCherry-LC3#21
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Kiyoshi Nobori, Akita University Graduate School of Medicine in 2008. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionThese mice express mCherry-LC3 in cardiomyocytes under the control of the alphaMyHC promoter.
Colony maintenance
References
Double transgenic mice crossed GFP-LC3 transgenic mice with alphaMyHC-mCherry-LC3 transgenic mice are a new and useful tool to examine the role of autophagy in the heart.
Terada M, Nobori K, Munehisa Y, Kakizaki M, Ohba T, Takahashi Y, Koyama T, Terata Y, Ishida M, Iino K, Kosaka T, Watanabe H, Hasegawa H, Ito H
Circ. J. 74, 203-206 (2010). 19966504

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
LC3 Map1lc3b (rat)UNLC3
mCherry Red Fluorescent Protein (Discosoma sp.)UNmCherry mouse alpha-myosin heavy chain promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse alpha-myosin heavy chain promoter, human Growth horman polyA signal, rat LC3 cDNA, Discosoma sp. mCherry cDNA
    Research applicationCell Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Circ J. 74, 203-206 (2010). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
    DepositorKiyoshi Nobori (Akita University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: FP license of TBUSA
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month May 2011

    BRC mice in Publications

    No Data