Strain Data Sheet

RBRC03928

Strain Information

Image
BRC No.RBRC03928
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Psmb11<tm1Smta>
Former Common namebeta5t KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6
Appearance
Strain development
Strain description
Colony maintenance
References
Regulation of CD8+ T cell development by thymus-specific proteasomes.
Murata S, Sasaki K, Kishimoto T, Niwa S, Hayashi H, Takahama Y, Tanaka K
Science, 316, 1349-1353 (2007). 17540904

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Psmb11
MGI:1921152		proteasome (prosome, macropain) subunit, beta type, 1114Psmb11<tm1Smta>
MGI:3716870		targeted mutation 1, Shigeo Murata
Venus Yellow Fluorescent Protein (Aequorea victoria)14Venus
neo neomycin resistance gene (E. coli)14
SV40 polyA signal14

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE.coli Neomycin resistance gene, MC1 promoter, Venus (a derivative of GFP) gene, SV40 PolyA signal
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 316, 1349-1353 (2007). In publishing the research results obtained by used of the biological resource, the recipient must describe the depositor as a coauthor of the study in the first publication only. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorShigeo Murata (Tokyo Metropolitan Institute of Medical Science)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ishii Y, Fukui-Miyazaki A, Iwasaki S, Tsuji T, Hotta K, Sasaki H, Nakagawa S, Yoshida T, Murata E, Taniguchi K, Shinohara N, Ishizu A, Kasahara M, Tomaru U.
    Impaired immunoproteasomal function exacerbates renal ischemia-reperfusion injury.
    Exp Mol Pathol 140 104939(2024) 39426027
    Tomaru U, Konno S, Miyajima S, Kimoto R, Onodera M, Kiuchi S, Murata S, Ishizu A, Kasahara M.
    Restricted Expression of the Thymoproteasome Is Required for Thymic Selection and Peripheral Homeostasis of CD8+ T Cells.
    Cell Rep 26(3) 639-651.e2(2019) 30650357