Strain Data Sheet

RBRC03934

Strain Information

Image
BRC No.RBRC03934
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(Ins1-cre)25Utr/Rbrc
Former Common nameInsulin-NLSCre
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResrouce Center in 2009. The transgene was injected into the pronuclei of C57BL/6N fertilized eggs.
Strain descriptionBAC Ins1-Cre transgenic mice. The mice were generated by using a BAC clone containing the mouse Ins1 gene, PR23-181I21. The Cre gene was inserted by homologous recombination into the second exon with a translational initiation codon in the Ins1 BAC clone. 6 founder lines were generated. The R26R crossing test indicated that line 25 (RBRC03934) is useful cre-driver for pancreatic beta cell-specific Cre-loxP recombination.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown).
References
Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination.
Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Mizuno-Iijima S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K
Exp. Anim., 62, 295-304 (2013). 24172193

Generation and characterization of Ins1-cre-driver C57BL/6N for exclusive pancreatic beta cell-specific Cre-loxP recombination.
Hasegawa Y, Daitoku Y, Mizuno S, Tanimoto Y, Mizuno-Iijima S, Matsuo M, Kajiwara N, Ema M, Oishi H, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K
Exp. Anim., 63(2):183-191 (2014). 24770644

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteUNFrt
cre Phage P1 Cre recombinaseUNcre
loxP phage P1 loxPUNloxP
nls SV40 large T antigen nuclear localization signal (NLS)UNnls mouse Insulin promoter (beta cell specific promoter region)
SV40 polyAUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 cre recombinase, yeast FRT (flippase recombination target) site, mouse Insulin promoter (beta cell specific promoter region), SV40 polyA, SV40 nuclear location signal(nls), Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-181I21
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 63(2):183-191 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ueki K, Nishida Y, Aoyama S, Uzawa H, Kanai A, Ito M, Ikeda K, Iida H, Miyatsuka T, Watada H.
    Establishment of Pancreatic Beta Cell-specific Gene Knockout System Based on CRISPR-Cas9 Technology with AAV8-mediated gRNA Delivery.
    Diabetes (2023) 37625131