Strain Data Sheet

RBRC03941

Strain Information

Image
BRC No.RBRC03941
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Sqstm1<tm2(GFP)Keta>/Rbrc
Former Common name
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain development
Strain description
Colony maintenance
References
Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells.
Eino A, Kageyama S, Uemura T, Annoh H, Saito T, Narita I, Waguri S, Komatsu M
J. Cell Sci., 128(23), 4453-4461 (2015). 26483381

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)11
Sqstm1
MGI:107931		sequestosome 111Sqstm1<tm2(GFP)Keta> targeted mutation 2, Keiji Tanaka
  • frontotemporal dementia and/or amyotrophic lateral sclerosis 3(MedGEN)

  • myopathy, distal, with rimmed vacuoles(MedGEN)

  • neurodegeneration with ataxia, dystonia, and gaze palsy, childhood-onset(MedGEN)
    • more 1 Diseases
  • Paget disease of bone 3(MedGEN)
    • neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    SV40 polyA11

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • enlarged liver(MP:0000599)

  • liver failure(MP:0003326)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Sqstm1(exon 3-8) cDNA, Jellyfish GFP cDNA, SV40 polyA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Sqstm1 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Keiji Tanaka, Tokyo Metropolitan Institute of Medical Science, Dr. Masaaki Komatsu, Niigata University and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature is requested. J. Cell Sci., 128(23), 4453-4461 (2015). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit oraganization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Eino A, Kageyama S, Uemura T, Annoh H, Saito T, Narita I, Waguri S, Komatsu M.
    Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells.
    J Cell Sci 128(23) 4453-61(2015) 26483381