Strain Data Sheet

RBRC03950

Strain Information

Image
BRC No.RBRC03950
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Psmb8<tm1Hjf>
Former Common nameLMP7 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Hans J. Fehling, Basel Institute for Immunology in 1995.
Strain description
Colony maintenance
References
MHC class I expression in mice lacking the proteasome subunit LMP-7.
Fehling H J, Swat W, Laplace C, Kuhn R, Rajewsky K, Muller U, von Boehmer H
Science, 265, 1234-1237 (1994). 8066463

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Psmb8
MGI:1346527		proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7)17Psmb8<tm1Hjf> targeted mutation 1, Hans Jorg Fehling
  • proteasome-associated autoinflammatory syndrome 1(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal dendritic cell antigen presentation(MP:0002455)

  • abnormal tumor susceptibility(MP:0002166)

  • decreased level of surface class I molecules(MP:0001841)

  • defective intracellular transport of class I molecules(MP:0001838)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, hsv tk polyA signal, mouse LMP7 gene cDNA
    Research applicationCell Biology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
    DepositorHajime Hisaeda (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Tomaru U, Konno S, Miyajima S, Kimoto R, Onodera M, Kiuchi S, Murata S, Ishizu A, Kasahara M.
    Restricted Expression of the Thymoproteasome Is Required for Thymic Selection and Peripheral Homeostasis of CD8+ T Cells.
    Cell Rep 26(3) 639-651.e2(2019) 30650357