Strain Data Sheet

RBRC03977

Strain Information

Image
BRC No.RBRC03977
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Chr5-Pirc<tm1Ms>
Former Common nameChr5 piRNA cluster KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Toshiaki Watabe and Yasuhiro Yamamoto, National Institute of Genetics in 2009. R1 ES cells were used.
Strain descriptionChromosome 5 piRNA cluster knockout mice. The promoter region of RNA precursor was replaced with a neo cassette. piRNA from Chr5 piRNA cluster was not generated. So far homozygous mutant mice show no obvious abnormality.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Chr5-Pirc Chromosome 5 piRNA cluster5Chr5-Pirc<tm1Ms> targeted mutation 1, National Institute of Genetics
GH Growth hormone polyA (Bovine)5GH
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter, bovine Growth hormone polyadenylation signal, P1 phage loxP
    Research applicationCell Biology Research
    Cre/loxP system
    Reproductive Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR and DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For two years form deposit, the RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR and DEVELOPER.
    DepositorHiroyuki Sasaki (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data