Strain Data Sheet

RBRC03980

Strain Information

Image
BRC No.RBRC03980
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Pld6<tm1.1Hsas>/MsRbrc
Former Common nameZuc KO, MitoPLD KO, B6;129-Zuc<tm1.1Ms>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Drs. Toshiaki Watabe and Hiroyuki Sasaki, National Institute of Genetics in 2009.
Strain description
Colony maintenance
References
MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline.
Watanabe T, Chuma S, Yamamoto Y, Kuramochi-Miyagawa S, Totoki Y, Toyoda A, Hoki Y, Fujiyama A, Shibata T, Sado T, Noce T, Nakano T, Nakatsuji N, Lin H, Sasaki H
Dev. Cell, 20 (3) 364-375 (2011). 21397847

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pld6
MGI:2687283		phospholipase D family member 611Pld6<tm1.1Hsas>
MGI:4946932		targeted mutation 1.1, Hiroyuki Sasaki
loxP phage P1 loxP11loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal DNA methylation during gametogenesis(MP:0008878)

  • abnormal male germ cell apoptosis(MP:0008280)

  • abnormal male germ cell morphology(MP:0006362)

  • abnormal spermatid morphology(MP:0006380)

  • abnormal spermatocyte morphology(MP:0006379)
    • more 5 phenotypes
  • abnormal testis physiology(MP:0004884)

  • arrest of male meiosis(MP:0008261)

  • azoospermia(MP:0005159)

  • male infertility(MP:0001925)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP site, mouse Pld6 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Reproductive Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR and DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For two years form deposit, the RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR and DEVELOPER.
    DepositorHiroyuki Sasaki (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data