Strain Data Sheet

RBRC03987

Strain Information

Image
BRC No.RBRC03987
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Ep400<tm3Fuku>
Former Common namemDomEx15-flox
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Rikiro Fukubnaga, Kyoto University in 2008.
Strain description
Colony maintenance
References
Critical role of the p400/mDomino chromatin-remodeling ATPase in embryonic hematopoiesis.
Ueda T, Watanabe-Fukunaga R, Ogawa H, Fukuyama H, Higashi Y, Nagata S, Fukunaga R
Genes Cells, 12, 581-592 (2007). 17535249

Essential role of p400/mDomino chromatin-remodeling ATPase in bone marrow hematopoiesis and cell-cycle progression.
Fujii T, Ueda T, Nagata S, Fukunaga R
J. Biol. Chem., 285(39):30214-30223(2010). 20610385

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ep400
MGI:1276124
E1A binding protein p4005Ep400<tm3Fuku>
MGI:4845874
targeted mutation 3, Rikiro Fukunaga
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal bone marrow cell morphology/development(MP:0002398)

  • abnormal bone marrow cell physiology(MP:0009278)

  • abnormal definitive hematopoiesis(MP:0002123)

  • abnormal megakaryocyte progenitor cell morphology(MP:0002413)

  • decreased bone marrow cell number(MP:0000333)
  • more 3 phenotypes
  • decreased erythroid progenitor cell number(MP:0008973)

  • decreased hematopoietic stem cell number(MP:0004810)

  • premature death(MP:0002083)
  • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse Ep400 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 285(39):30214-30223(2010). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author.
    DepositorRikiro Fukunaga (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Contact Information (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Fröb F, Sock E, Tamm ER, Saur AL, Hillgärtner S, Williams TJ, Fujii T, Fukunaga R, Wegner M.
    Ep400 deficiency in Schwann cells causes persistent expression of early developmental regulators and peripheral neuropathy.
    Nat Commun 10(1) 2361(2019) 31142747
    Elsesser O, Fröb F, Küspert M, Tamm ER, Fujii T, Fukunaga R, Wegner M.
    Chromatin remodeler Ep400 ensures oligodendrocyte survival and is required for myelination in the vertebrate central nervous system.
    Nucleic Acids Res 47(12) 6208-6224(2019) 31081019
    Fujii T, Ueda T, Nagata S, Fukunaga R.
    Essential role of p400/mDomino chromatin-remodeling ATPase in bone marrow hematopoiesis and cell-cycle progression.
    J Biol Chem 285(39) 30214-23(2010) 20610385