Strain Data Sheet

RBRC04109

Strain Information

Image
BRC No.RBRC04109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Calca<tm1Hku>
Former Common namealphaCGRP-
H-2 Haplotype
ES Cell lineSM1[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Hiroki Kurihara, Graduate School of Medicine, University of Tokyo in 2001.
Strain descriptionThe alpha-Calcitonin gene-related peptide (alphaCGRP) sequence was disrupted by replacement of exon 5 with a PGK-neo cassette leaving the calcitonin (CT) sequence uninterrupted. RT-PCR analysis confirmed the absence of alphaCGRP expression in mutant mice while CT expression appears unaffected. Immunohistochemical analysis did not detect alphaCGRP protein in the spinal cords of mutant animals, but thyroid gland samples stain positive for CT. The elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability.
Colony maintenanceThe elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability.
References
Elevated sympathetic nervous activity in mice deficient in alphaCGRP.
Oh-hashi Y, Shindo T, Kurihara Y, Imai T, Wang Y, Morita H, Imai Y, Kayaba Y, Nishimatsu H, Suematsu Y, Hirata Y, Yazaki Y, Nagai R, Kuwaki T, Kurihara H
Circ. Res., 89, 983-990 (2001). 11717154

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Calca
MGI:2151253		calcitonin/calcitonin-related polypeptide, alpha7Calca
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal sympathetic nervous system physiology(MP:0004183)

  • abnormal urine catecholamine level(MP:0011478)

  • decreased airway responsiveness(MP:0002335)

  • decreased heart rate variability(MP:0003929)

  • increased heart rate(MP:0002626)
    • more 2 phenotypes
  • increased mean systemic arterial blood pressure(MP:0004875)

  • increased systemic vascular resistance(MP:0004878)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Calca genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Circ res., 89, 983-990 (2001).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorHiroki Kurihara (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Hashikawa-Hobara N, Inoue S, Hashikawa N.
    Lack of alpha CGRP exacerbates the development of atherosclerosis in ApoE-knockout mice.
    Sci Rep 14(1) 18377(2024) 39112593
    Jiang Y, Yuan Y, Xiong Y, Wang B, Guo Y, Gong P, Zhang L.
    Low-intensity pulsed ultrasound improves osseointegration of dental implant in mice by inducing local neuronal production of αCGRP.
    Arch Oral Biol 115 104736(2020) 32371135
    Wang B, Lin J, Zhang Q, Zhang X, Yu H, Gong P, Xiang L.
    αCGRP Affects BMSCs' Migration and Osteogenesis via the Hippo-YAP Pathway.
    Cell Transplant 28(11) 1420-1431(2019) 31426665
    Xiang L, Ma L, Wei N, Wang T, Yao Q, Yang B, Xiong Y, Wu Y, Gong P.
    Effect of lentiviral vector overexpression α-calcitonin gene-related peptide on titanium implant osseointegration in α-CGRP-deficient mice.
    Bone 94 135-140(2017) 26265538