Strain Data Sheet

RBRC04111

Strain Information

Image
BRC No.RBRC04111
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(NDE-hsp-Pkd2,lacZ)34Hmd
Former Common nameNDE-Pkd2-IZ(34)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2000. Transgene was injected to into the pronuclei of (C57BL/6 x C3H) F1 fertilized eggs. The mice were crossed to B6/129 background strain.
Strain descriptionThese transgenic mice express Pkd2/lacZ gene on both sides of the node under the control of the node specific enhancer.
Colony maintenance
References
Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2.
Yoshiba S, Shiratori H, Kuo I Y, Kawasumi A, Shinohara K, Nonaka S, Asai Y, Sasaki G, Belo J A, Sasaki H, Nakai J, Dworniczak B, Ehrlich B E, Pennekamp P, Hamada H
Science, 338, 226-231 (2012). 22983710

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRES internal ribosomal entry site (EMCV)UN
Pkd2 polycystic kidney disease 2UNPkd2 mouse node-specific enhancer, hsp promoter
lacZ beta-galactosidase (E. coli)UN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAマウス由来Nodalのノードエンハンサー, Pkd2cDNA, hspプロモーター, E.coli由来LacZ遺伝子, ウィルス由来IRES配列
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 338, 226-231 (2012).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data