Strain Data Sheet

RBRC04112

Strain Information

Image
BRC No.RBRC04112
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Nodal<tm2Hmd>
Former Common nameNodal beta geo
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2004.
Strain description
Colony maintenance
References
Both Nodal signalling and stochasticity select for prospective distal visceral endoderm in mouse embryos.
Katsuyoshi Takaoka, Hiromi Nishimura, Hiroshi Hamada
Nat. Commun., 8(1):1492 (2017). 29138408

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site10Frt
Ires internal ribosomal entry site (EMCV)10
Nodal
MGI:97359		nodal growth differentiation factor10Nodal<tm2Hmd> targeted mutation 2, Hiroshi Hamada
lacZ beta-galactosidase (E. coli)10
loxP phage P1 loxP10loxP
neo neomycin resistance gene (E. coli)10

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE coli neo, E. coli lacZ, phage P1 loxP, yeast frt, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires)
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 8(1):1492. (2017).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data