Strain Data Sheet

RBRC04113

Strain Information

Image
BRC No.RBRC04113
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Nodal<tm2.1Hmd>
Former Common name
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2004.
Strain description
Colony maintenance
References
Both Nodal signalling and stochasticity select for prospective distal visceral endoderm in mouse embryos.
Katsuyoshi Takaoka, Hiromi Nishimura, Hiroshi Hamada
Nat. Commun., 8(1):1492 (2017). 29138408

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site10Frt
Nodal
MGI:97359		nodal growth differentiation factor10Nodal<tm2.1Hmd> targeted mutation 2.1, Hiroshi Hamada
loxP phage P1 loxP10loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAbacteriophage由来 loxP配列, yeast由来 frt配列
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 8(1):1492. (2017).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data