Strain Data Sheet

RBRC04197

Strain Information

Image
BRC No.RBRC04197
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cxcr4<tm1Tng>
Former Common nameCXCR4 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Takashi Nagasawa, Resrarch institute, Osaka Medical Center for Maternal and Child Health.
Strain description
Colony maintenance
References
The chemokine receptor CXCR4 is essential for vascularization of the gastrointestinal tract.
Tachibana K, Hirota S, Iizasa H, Yoshida H, Kawabata K, Kataoka Y, Kitamura Y, Matsushima K, Yoshida N, Nishikawa S, Kishimoto T, Nagasawa T
Nature, 393, 591-594 (1998). 9634237

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cxcr4
MGI:109563		C-X-C motif chemokine receptor 41Cxcr4<tm1Tng>
MGI:1934442		targeted mutation 1, Takashi Nagasawa
  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)
    • neo neomycin resistance gene (E. coli)1 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell differentiation(MP:0002144)

  • abnormal T cell differentiation(MP:0002145)

  • abnormal angiogenesis(MP:0000260)

  • abnormal blood vessel morphology(MP:0001614)

  • abnormal developmental vascular remodeling(MP:0009577)
    • more 19 phenotypes
  • abnormal heart morphology(MP:0000266)

  • abnormal heart right ventricle outflow tract morphology(MP:0010428)

  • abnormal primordial germ cell migration(MP:0002982)

  • abnormal vascular smooth muscle morphology(MP:0005592)

  • abnormal vasculogenesis(MP:0001622)

  • cardiovascular system phenotype(MP:0005385)

  • decreased double-positive T cell number(MP:0005092)

  • decreased plasma cell number(MP:0008098)

  • digestive/alimentary phenotype(MP:0005381)

  • embryo phenotype(MP:0005380)

  • failure of myelopoiesis(MP:0001603)

  • gastrointestinal hemorrhage(MP:0000465)

  • neonatal lethality, complete penetrance(MP:0011087)

  • nervous system phenotype(MP:0003631)

  • perimembraneous ventricular septal defect(MP:0010418)

  • perinatal lethality, incomplete penetrance(MP:0011090)

  • poor arterial differentiation(MP:0000262)

  • thick mitral valve cusps(MP:0010617)

  • ventricular septal defect(MP:0010402)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHSV thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, HSV thymidine kinase polyA signal, Mouse Cxcr4 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
    DepositorTakashi Nagasawa (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data