Strain Data Sheet

RBRC04199

Strain Information

Image
BRC No.RBRC04199
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cxcl12<tm1Tng>
Former Common nameCXCL12 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Takashi Nagasawa, Resrarch institute, Osaka Medical Center for Maternal and Child Health.
Strain description
Colony maintenance
References
Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1.
Nagasawa T, Hirota S, Tachibana K, Takakura N, Nishikawa S, Kitamura Y, Yoshida N, Kikutani H, Kishimoto T
Nature, 382, 635-638 (1996). 8757135

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cxcl12
MGI:103556
C-X-C motif chemokine ligand 126Cxcl12<tm1Tng>
MGI:1934384
targeted mutation 1, Takashi Nagasawa
  • susceptibility to HIV infection(MedGEN)
  • neo neomycin resistance gene (E. coli)6 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal angiogenesis(MP:0000260)

  • abnormal blood cell morphology/development(MP:0002429)

  • abnormal blood vessel morphology(MP:0001614)

  • abnormal bone marrow cell physiology(MP:0009278)

  • abnormal cerebellum morphology(MP:0000849)
  • more 20 phenotypes
  • abnormal common lymphocyte progenitor cell morphology(MP:0008249)

  • abnormal developmental vascular remodeling(MP:0009577)

  • abnormal hematopoietic stem cell physiology(MP:0010763)

  • abnormal primordial germ cell migration(MP:0002982)

  • abnormal vascular smooth muscle morphology(MP:0005592)

  • decreased B cell number(MP:0005017)

  • decreased bone marrow cell number(MP:0000333)

  • decreased common myeloid progenitor cell number(MP:0008813)

  • decreased hematopoietic stem cell number(MP:0004810)

  • decreased pre-B cell number(MP:0008209)

  • decreased pre-pro B cell number(MP:0011816)

  • decreased pro-B cell number(MP:0008208)

  • hematopoietic system phenotype(MP:0005397)

  • impaired myelopoiesis(MP:0001602)

  • lethality throughout fetal growth and development, incomplete penetrance(MP:0011109)

  • neonatal lethality, complete penetrance(MP:0011087)

  • nervous system phenotype(MP:0003631)

  • perimembraneous ventricular septal defect(MP:0010418)

  • poor arterial differentiation(MP:0000262)

  • thin external granule cell layer(MP:0000873)
  • Detailed phenotype data

    Ordering Information

    Donor DNAHSV thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, HSV thymidine kinase polyA signal, Mouse Cxcl12 genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
    DepositorTakashi Nagasawa (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Greenbaum A, Hsu YM, Day RB, Schuettpelz LG, Christopher MJ, Borgerding JN, Nagasawa T, Link DC.
    CXCL12 in early mesenchymal progenitors is required for haematopoietic stem-cell maintenance.
    Nature 495(7440) 227-30(2013) 23434756