Strain Data Sheet

RBRC04200

Strain Information

Image
BRC No.RBRC04200
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cxcl12<tm2Tng>
Former Common nameCXCL12-GFP
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Takashi Nagasawa, Research Institute, Osaka Medical Center for Maternal and Child Health. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6J for 6 times, and then maintained by sibling mating.
Strain descriptionCXCL12-GFP knock-in mice. Exon 1 of the Cxcl12 gene was replaced with a GFP expression cassette.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJcl]
References
Long-term hematopoietic stem cells require stromal cell-derived factor-1 for colonizing bone marrow during ontogeny.
Ara T, Tokoyoda K, Sugiyama T, Egawa T, Kawabata K, Nagasawa T
Immunity, 19, 257-267 (2003). 12932359

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cxcl12
MGI:103556		C-X-C motif chemokine ligand 126Cxcl12<tm2Tng>
MGI:3579534		targeted mutation 2, Takashi Nagasawa
  • susceptibility to HIV infection(MedGEN)
    • EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP
    loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAJellyfish EGFP cDNA, SV40 PolyA signal, Phage P1 loxP sites, smouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Mouse Cxcl12 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
    DepositorTakashi Nagasawa (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Dec 2014

    BRC mice in Publications

    No Data