Strain Data Sheet

RBRC04287

Strain Information

Image
BRC No.RBRC04287
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain namePcdhg-DgHS1-5
Former Common nameDgHS1-5
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2009. TT2 ES cells were used.
Strain descriptionPcdhg knockout mice. The gHS1-5 genomic region of the Pcdhg gene was deleted. Diap1 gene was also deleted. About 30% homozygous mutant mice die within 3 days after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
Identification of the cluster control region for the protocadherin-beta genes located beyond the protocadherin-gamma cluster.
Yokota S, Hirayama T, Hirano K, Kaneko R, Toyoda S, Kawamura Y, Hirabayashi M, Hirabayashi T, Yagi T
J Biol Chem. , 286, 31885-31895 (2011). 21771796

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRES internal ribosomal entry site (EMCV)18
Pcdhg
MGI:3719124		protocadherin gamma cluster18Pcdhg<tm(gHS1-5/del)> targeted mutation
lacZ beta-galactosidase (E. coli)18
loxP phage P1 loxP18loxP
tau bovine tau protein cDNA18tau

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • postnatal lethality(MP:0002082)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Pcdh-g gene, P1 phage loxP, E. coli LacZ, Encephalomyocarditis virus ires (internal ribosone entry site), bovine tau
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data