Strain Data Sheet

RBRC04292

Strain Information

Image
BRC No.RBRC04292
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Pold1<tm1.uchi>
Former Common namePold1<tm1Uchi>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionPold1 (polymerase (DNA directed), delta 1, catalytic subunit) gene knockout mice. A loxP flanked polyA-neo cassette was inserted in intron 10. The exon 10 contains a D400A substitution. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
DNA polymerase delta is required for early mammalian embryogenesis.
Uchimura A, Hidaka Y, Hirabayashi T, Hirabayashi M, Yagi T
PLoS One, 4, e4184 (2009). 19145245

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pold1
MGI:97436		olfactory marker protein7Pold1<tm1Uchi>
MGI:3833573		targeted mutation 1, Arikuni Uchimura
loxP phage P1 loxP7loxP
loxP phage P1 loxP7loxP
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal7

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal trophoblast layer morphology(MP:0005031)

  • absent inner cell mass proliferation(MP:0012114)

  • embryo phenotype(MP:0005380)

  • embryonic lethality between implantation and somite formation, complete penetrance(MP:0011096)

  • enlarged thymus(MP:0000709)
    • more 3 phenotypes
  • increased tumor incidence(MP:0002020)

  • inner cell mass degeneration(MP:0004965)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), SV40 PolyA signal, P1 phage loxP, E. coli neo, mouse Pold1 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data