Strain Information | |
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Image | |
BRC No. | RBRC04293 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6-Pold1<exo.uchi> |
Former Common name | Pold1<exo.Uchi> |
H-2 Haplotype | |
ES Cell line | Not specified (ES cell) |
Background strain | |
Appearance | |
Strain development | Developed by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The polyA-neo cassette was removed by crossing with Sycp1-cre mice. The mutant mice were crossed to C57BL/6J. |
Strain description | Pold1 (polymerase (DNA directed) D400A variant mutant mice lacking 3’-5’exonuclease activity in the Pol delta. About 30% of homozygous mutant mice die with abnormally swollen thymus. Most surviving mice develop nodules on their tails. It is preferable to avoid a mating using heterozygous male across continuous generations (2 generations and up) to avoid an increasing mutation rate. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6J(Crlj)] |
References | DNA polymerase delta is required for early mammalian embryogenesis. Uchimura A, Hidaka Y, Hirabayashi T, Hirabayashi M, Yagi T PLoS One, 4, e4184 (2009). 19145245Germline mutation rates and the long-term phenotypic effects of mutation accumulation in wild-type laboratory mice and mutator mice. Uchimura A, Higuchi M, Minakuchi Y, Ohno M, Toyoda A, Fujiyama A, Miura I, Wakana S, Nishino J, Yagi T Genome Res., 25(8)1125-1134 (2015). 26129709 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Pold1 MGI:97436 | olfactory marker protein | 7 | Pold1<tm1.1Uchi> MGI:3833575 | targeted mutation 1.1, Arikuni Uchimura | |||
loxP | phage P1 loxP | 7 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | P1 phage loxP site, Mouse Pold1 genomic DNA |
Research application | Cell Biology Research Cre/loxP system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. |
Depositor | Takeshi Yagi (Osaka University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Dec 2015 |
BRC mice in Publications |
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No Data |