Strain Data Sheet

RBRC04293

Strain Information

Image
BRC No.RBRC04293
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Pold1<exo.uchi>
Former Common namePold1<exo.Uchi>
H-2 Haplotype
ES Cell lineNot specified (ES cell)
Background strain
Appearance
Strain developmentDeveloped by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The polyA-neo cassette was removed by crossing with Sycp1-cre mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionPold1 (polymerase (DNA directed) D400A variant mutant mice lacking 3’-5’exonuclease activity in the Pol delta. About 30% of homozygous mutant mice die with abnormally swollen thymus. Most surviving mice develop nodules on their tails. It is preferable to avoid a mating using heterozygous male across continuous generations (2 generations and up) to avoid an increasing mutation rate.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
DNA polymerase delta is required for early mammalian embryogenesis.
Uchimura A, Hidaka Y, Hirabayashi T, Hirabayashi M, Yagi T
PLoS One, 4, e4184 (2009). 19145245

Germline mutation rates and the long-term phenotypic effects of mutation accumulation in wild-type laboratory mice and mutator mice.
Uchimura A, Higuchi M, Minakuchi Y, Ohno M, Toyoda A, Fujiyama A, Miura I, Wakana S, Nishino J, Yagi T
Genome Res., 25(8)1125-1134 (2015). 26129709

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pold1
MGI:97436
olfactory marker protein7Pold1<tm1.1Uchi>
MGI:3833575
targeted mutation 1.1, Arikuni Uchimura
loxP phage P1 loxP7loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • embryo phenotype(MP:0005380)

  • enlarged thymus(MP:0000709)

  • increased tumor incidence(MP:0002020)

  • premature death(MP:0002083)
  • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP site, Mouse Pold1 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Dec 2015

    BRC mice in Publications

    No Data