Strain Data Sheet

RBRC04333

Strain Information

Image
BRC No.RBRC04333
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Cyp26b1<tm2Hmd>
Former Common nameCyp26b1 flox
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2004.
Strain descriptionCyp26b1 conditional knockout mice. A loxP site is inserted into intron 4 and 3’ UTR, respectively. Conditional Cyp26b1 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance
References
Increased retinoic acid levels through ablation of Cyp26b1 determine the processes of embryonic skin barrier formation and peridermal development.
Okano J, Lichti U, Mamiya S, Aronova M, Zhang G, Yuspa S H, Hamada H, Sakai Y, Morasso M I
J. Cell Sci., 125(Pt 7):1827-36 (2012). 22366455

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cyp26b1
MGI:2176159		cytochrome P450, family 26, subfamily b, polypeptide 16Cyp26b1<tm2Hmd> targeted mutation 2, Hiroshi Hamada
  • lethal occipital encephalocele-skeletal dysplasia syndrome(MedGEN)
    • Frt yeast FRT (flippase recombination target) site6Frt
    loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal apoptosis(MP:0001648)

  • abnormal corneocyte envelope morphology(MP:0009594)

  • abnormal epidermal layer morphology(MP:0001216)

  • abnormal epidermis stratum basale morphology(MP:0001231)

  • abnormal epidermis stratum corneum morphology(MP:0001240)
    • more 16 phenotypes
  • abnormal forelimb morphology(MP:0000550)

  • abnormal hair follicle development(MP:0003704)

  • abnormal hindlimb morphology(MP:0000556)

  • abnormal limb bone morphology(MP:0002115)

  • abnormal limb morphology(MP:0002109)

  • abnormal lymphatic vessel endothelial cell morphology(MP:0010197)

  • abnormal proximal-distal axis patterning(MP:0001705)

  • absent keratohyalin granules(MP:0009603)

  • delayed chondrocyte differentiation(MP:0014106)

  • eyelids open at birth(MP:0001302)

  • impaired skin barrier function(MP:0002796)

  • micrognathia(MP:0002639)

  • neonatal lethality, complete penetrance(MP:0011087)

  • oligodactyly(MP:0000565)

  • respiratory distress(MP:0001954)

  • short limbs(MP:0000547)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP sites, yeast FRT (flipase recombination target) site, mouse Cyp26b1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 125(Pt 7):1827-36 (2012).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Schönberger K, Obier N, Romero-Mulero MC, Cauchy P, Mess J, Pavlovich PV, Zhang YW, Mitterer M, Rettkowski J, Lalioti ME, Jäcklein K, Curtis JD, Féret B, Sommerkamp P, Morganti C, Ito K, Ghyselinck NB, Trompouki E, Buescher JM, Pearce EL, Cabezas-Wallscheid N.
    Multilayer omics analysis reveals a non-classical retinoic acid signaling axis that regulates hematopoietic stem cell identity.
    Cell Stem Cell 29(1) 131-148.e10(2022) 34706256