Strain Information | |
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Image | |
BRC No. | RBRC04364 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.Cg-Aqp4<tm1.1(GFP)Mysi>/5Rbrc |
Former Common name | Aquaporin-4 (AQP4) knockout mouse, AQP4 null mouse, aquaporin4 (GFP), aquaporin 4(GFP) (Acc.No. CDB0758K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Masato Yasui, School of Medicine, Keio University/Johns Hopkins University and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology. |
Strain description | aquaporin 4 gene GFP knock-in mice. EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04397, BALB/c background: RBRC04399), delta Pgk-neo type (#5 ES clone derived)(B6 background: RBRC04364, BALB/c mixed: RBRC04400), delta EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04401, BALB/c mixed: RBRC04402), EGFP-Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04398), delta Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04403). |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin. Ikeshima-Kataoka H, Abe Y, Abe T, Yasui M Mol Cell Neurosci, 56, 65-75 (2013). 23428384Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke. Monai H, Wang X, Yahagi K, Lou N, Mestre H, Xu Q, Abe Y, Yasui M, Iwai Y, Nedergaard M, Hirase H Proc. Natl. Acad. Sci. USA, 116(22):11010-11019 (2019). 31097598 |
Health Report | |
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Examination Date / Room / Rack | 2024/12/09Room:3-3Rack:DSentinel mouse program 2024/09/09Room:3-3Rack:DSentinel mouse program 2024/06/10Room:3-3Rack:DSentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Aqp4 MGI:107387 | aquaporin 4 | 18 | Aqp4<tm1.1(GFP)> | targeted mutation 1.1Mysi | |||
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 18 | EGFP | ||||
Frt | yeast FRT (flippase recombination target) site | 18 | Frt | ||||
loxP | phage P1 loxP | 18 | loxP | ||||
loxP | phage P1 loxP | 18 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, Jellyfish EGFP cDNA, yeast FRT (flipase recombination target) site, Mouse polyA signal, mouse Aqp4 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0758K-1) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Mol Cell Neurosci, 56, 65-75 (2013). ) (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Michael Nicosia, Juyeun Lee, Ashley Beavers, Danielle Kish, George W Farr, Paul R McGuirk, Marc F Pelletier, Justin D Lathia, Robert L Fairchild, Anna Valujskikh Water channel Aquaporin 4 is required for T cell receptor mediated lymphocyte activation Journal of Leukocyte Biology (2023) 36805947 |
Nicosia M, Miyairi S, Beavers A, Farr GW, McGuirk PR, Pelletier MF, Valujskikh A. Aquaporin 4 inhibition alters chemokine receptor expression and T cell trafficking. Sci Rep 9(1) 7417(2019) 31092872 |
Z Heřmanová The Role of Aquaporin 4 channels and Transient Receptor Potential Vanilloid 4 channels in astrocytic swelling. Univerzita Karlova (2019) |