Strain Data Sheet

RBRC04364

Strain Information

Image
BRC No.RBRC04364
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aqp4<tm1.1(GFP)Mysi>/5Rbrc
Former Common nameAquaporin-4 (AQP4) knockout mouse, AQP4 null mouse, aquaporin4 (GFP), aquaporin 4(GFP) (Acc.No. CDB0758K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentMasato Yasui, School of Medicine, Keio University/Johns Hopkins University and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology.
Strain descriptionaquaporin 4 gene GFP knock-in mice. EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04397, BALB/c background: RBRC04399), delta Pgk-neo type (#5 ES clone derived)(B6 background: RBRC04364, BALB/c mixed: RBRC04400), delta EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04401, BALB/c mixed: RBRC04402), EGFP-Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04398), delta Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04403).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin.
Ikeshima-Kataoka H, Abe Y, Abe T, Yasui M
Mol Cell Neurosci, 56, 65-75 (2013). 23428384

Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke.
Monai H, Wang X, Yahagi K, Lou N, Mestre H, Xu Q, Abe Y, Yasui M, Iwai Y, Nedergaard M, Hirase H
Proc. Natl. Acad. Sci. USA, 116(22):11010-11019 (2019). 31097598

Health Report

Examination Date / Room / Rack2024/12/09Room:3-3Rack:DSentinel mouse program
2024/09/09Room:3-3Rack:DSentinel mouse program
2024/06/10Room:3-3Rack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aqp4
MGI:107387
aquaporin 418Aqp4<tm1.1(GFP)> targeted mutation 1.1Mysi
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)18EGFP
Frt yeast FRT (flippase recombination target) site18Frt
loxP phage P1 loxP18loxP
loxP phage P1 loxP18loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal microglial cell physiology(MP:0003691)

  • cerebral edema(MP:0011854)
  • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, Jellyfish EGFP cDNA, yeast FRT (flipase recombination target) site, Mouse polyA signal, mouse Aqp4 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0758K-1) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Mol Cell Neurosci, 56, 65-75 (2013). ) (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Michael Nicosia, Juyeun Lee, Ashley Beavers, Danielle Kish, George W Farr, Paul R McGuirk, Marc F Pelletier, Justin D Lathia, Robert L Fairchild, Anna Valujskikh
    Water channel Aquaporin 4 is required for T cell receptor mediated lymphocyte activation
    Journal of Leukocyte Biology (2023) 36805947
    Nicosia M, Miyairi S, Beavers A, Farr GW, McGuirk PR, Pelletier MF, Valujskikh A.
    Aquaporin 4 inhibition alters chemokine receptor expression and T cell trafficking.
    Sci Rep 9(1) 7417(2019) 31092872
    Z Heřmanová
    The Role of Aquaporin 4 channels and Transient Receptor Potential Vanilloid 4 channels in astrocytic swelling.
    Univerzita Karlova (2019)