Strain Data Sheet

RBRC04371

Strain Information

Image
BRC No.RBRC04371
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Tdrd1<tm1Chum>
Former Common nameTdrd1 knock-out
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Shinichiro Chuma, Institute for Frontier Medical Sciences, Kyoto University.
Strain descriptionTdrd1 Knockout mice. Male homozygous mice are sterile, displaying postnatal spermatogenic defects. Females are fertile.
Colony maintenance
References
Tdrd1/Mtr-1, a tudor-related gene, is essential for male germ-cell differentiation and nuage/germinal granule formation in mice.
Chuma S, Hosokawa M, Kitamura K, Kasai S, Fujioka M, Hiyoshi M, Takamune K, Noce T, Nakatsuji N
Proc. Natl. Acad. Sci. USA, 103, 15894-15899 (2006). 17038506

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tdrd1
MGI:1933218		tudor domain containing 119Tdrd1<tm1Chum>
MGI:3691852		targeted mutation 1, Shinichiro Chuma
neo neomycin resistance gene (E. coli)19 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal male germ cell apoptosis(MP:0008280)

  • abnormal oocyte morphology(MP:0001125)

  • abnormal spermatid morphology(MP:0006380)

  • abnormal spermatocyte morphology(MP:0006379)

  • abnormal spermatogenesis(MP:0001156)
    • more 4 phenotypes
  • arrest of spermatogenesis(MP:0001155)

  • azoospermia(MP:0005159)

  • male infertility(MP:0001925)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, mouse Tdrd1 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR.
    DepositorShinichiro Chuma (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data