Strain Data Sheet

RBRC04372

Strain Information

Image
BRC No.RBRC04372
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129(SJL)-Tdrd9<tm1.1Chum>
Former Common nameTdrd9 knock-out
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain development
Strain descriptionMale homozygous mice are sterile, displaying small testis, arrest of male meiosis and abnormal spermatocyte morphology. Females are fertile.
Colony maintenance
References
The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.
Shoji M, Tanaka T, Hosokawa M, Reuter M, Stark A, Kato Y, Kondoh G, Okawa K, Chujo T, Suzuki T, Hata K, Martin S L, Noce T, Kuramochi-Miyagawa S, Nakano T, Sasaki H, Pillai R S, Nakatsuji N, Chuma S
Dev. Cell., 17, 775-787 (2009). 20059948

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tdrd9
MGI:1921941		tudor domain containing 912Tdrd9<tm1.1Chum>
MGI:4421849		targeted mutation 1, Shinichiro Chuma
  • spermatogenic failure 30(MedGEN)
    • loxP phage P1 loxP12loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal DNA methylation during gametogenesis(MP:0008878)

  • abnormal male germ cell apoptosis(MP:0008280)

  • abnormal male germ cell morphology(MP:0006362)

  • abnormal spermatocyte morphology(MP:0006379)

  • abnormal spermatogonia morphology(MP:0006378)
    • more 6 phenotypes
  • abnormal synaptonemal complex(MP:0009452)

  • arrest of male meiosis(MP:0008261)

  • azoospermia(MP:0005159)

  • decreased testis weight(MP:0004852)

  • male infertility(MP:0001925)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse tdrd9 genomic DNA, phage P1 loxP
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR.
    DepositorShinichiro Chuma (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data