Strain Data Sheet

RBRC04394

Strain Information

Image
BRC No.RBRC04394
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Lyz2<tm1(DTR)Mtka>
Former Common nameLysM-DTR KI, B6;129-Lyz2<tm1(DTR)Mtka>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloed by Masato Tanaka, RIKEN Research Center for Allergy and Immunology in 2007.
Strain description
Colony maintenance
References
Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice.
M Saito, T Iwawaki, C Taya, H Yonekawa, M Noda, Y Inui, E Mekada, Y Kimata, A Tsuru, K Kohno
Nat. Biotechnol., 19, 746-750 (2001). 11479567

Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice.
Saito M, Iwawaki T, Taya C, Yonekawa H, Noda M, Inui Y, Mekada E, Kimata Y, Tsuru A, Kohno K
Nat. Biotechnol., 19, 746-750 (2001). 11479567

Protective role of macrophages in noninflammatory lung injury caused by selective ablation of alveolar epithelial type II Cells.
Miyake Y, Kaise H, Isono K, Koseki H, Kohno K, Tanaka M
J. Immunol., 178, 5001-5009 (2007). 17404282

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2 rabbit beta-globin polyA10HBB2
HBEGF heparin-binding EGF-like growth factor (human)10HBEGF
Lyz2
MGI:96897		lysozyme 210Lyz2<tm1(DTR)Mtka>
MGI:3834185		targeted mutation 1, Masato Tanaka
  • familial visceral amyloidosis(MedGEN)
    • loxP phage P1 loxP10loxP
    loxP phage P1 loxP10loxP
    neo neomycin resistance gene (E. coli)10 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal alveolar macrophage morphology(MP:0008245)

  • abnormal liver morphology(MP:0000598)

  • abnormal lung interstitium morphology(MP:0002276)

  • abnormal pulmonary alveolus morphology(MP:0002270)

  • abnormal surfactant secretion(MP:0004780)
    • more 8 phenotypes
  • absent type II pneumocytes(MP:0010812)

  • decreased locomotor activity(MP:0001402)

  • hunched posture(MP:0001505)

  • increased lactate dehydrogenase level(MP:0002944)

  • lung hemorrhage(MP:0001182)

  • premature death(MP:0002083)

  • respiratory failure(MP:0001953)

  • ruffled hair(MP:0000420)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHuman HB-EGF(DTR) cDNA, rabbit beta-globin polyA (HBB2), Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistance gene, Mouse LysM genomic DNA (Update June 9 2023)
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Biotechnol., 19, 746-750 (2001). J. Immunol., 178, 5001-5009 (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Kenji Kohno, Ph.D., Masato Tanaka, Ph.D. The recipient shall obtain a prior written approval from the depositor.
    DepositorMasato Tanaka (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Apr 2011

    BRC mice in Publications

    No Data