Strain Data Sheet

RBRC04415

Strain Information

Image
BRC No.RBRC04415
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Lefty1-Cre/ERT2)8Hmd
Former Common nameLefty1-CreERT2/BAC (8)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kastuyoshi Takaoka and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2009.
Strain description
Colony maintenance
References
Origin and role of distal visceral endoderm, a group of cells that determines anterior-posterior polarity of the mouse embryo.
Takaoka K, Yamamoto M, Hamada H
Nat. Cell Biol., 13, 743-752 (2011). 21623358

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2
cre Phage P1 Cre recombinaseUNcre mouse lefty1 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Lefty1 genomic DNA from BAC clone RP23-390I1, Phage P1 Cre, human Estrogen receptor binding domain (ERT2) cDNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Cell Biol., 13, 743-752 (2011).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Tsubosaka A, Komura D, Kakiuchi M, Katoh H, Onoyama T, Yamamoto A, Abe H, Seto Y, Ushiku T, Ishikawa S.
    Stomach encyclopedia: Combined single-cell and spatial transcriptomics reveal cell diversity and homeostatic regulation of human stomach.
    Cell Rep 42(10) 113236(2023) 37819756